HPLC-ELSD测定小桐子生物柴油副产物甘油含量  

Determination of By-Product of Glycerol in Jatropha Biodiesel Oil by HPLC-ELSD

作　　者：张瑶[1] 李宝才[1] 陈浩[1] 刘存存[1] 杨宏博[1] 王耘 陈卓 

机构地区：[1]昆明理工大学生命科学与技术学院,昆明市白龙寺296号650224 [2]云南神宇新能源有限公司,昆明市650224

出　　处：《光谱实验室》2011年第5期2584-2589,共6页Chinese Journal of Spectroscopy Laboratory

基　　金：小桐子生物柴油副产品综合利用技术与产业化开发-医用甘油生产技术开发与应用任务[2007BAD32B04(5)]

摘　　要：建立高效液相色谱-蒸发光散射检测器(HPLC-ELSD)测定小桐子生物柴油副产物甘油含量的方法。以Interstil NH2(250mm×4.6mm,5μm)为色谱柱;乙腈-水(80∶20,V/V)为流动相;甘油进样量在0.016—4.096μg范围时与主峰面积有良好的线性关系,方法平均回收率在93.00%以上,精密度的相对标准偏差为<3.00%(n=5)。该方法简单,快速,灵敏度高,重复性好。A method for the determination of the content of the by-product of glycerine in jatropha biodiesel by high performance liquid chromatography-evaporative light scattering detector（HPLC-ELSD） was developed.With acetonitrile-H2O（80∶20） as mobile phase,the HPLC analysis was carried out on a Interstil NH2（250mm×4.6mm,5μm）column.There was a good linear relationship between sample size of glycerine and main peak areas in the range of 0.016—4.096μg.The recovery of this method was above 93.00%,and RSDs were less than 3.00%（n=5）.The method is simple,quick,sensitive and repeatable.

关 键 词：甘油 生物柴油 高效液相色谱 蒸发光散射检测器 

分 类 号：O657.72[理学—分析化学]