血小板膜糖蛋白（GP）Ibα胞内区551到565氨基酸序列对GPIbα结合VWF功能的调控作用  

The role of amino acid sequence between 551 and 565 in the cytoplasmic domain of glycoprotein （GP） I bα in the regulation of the VWF binding to GP I bα

作　　者：张卫林[1] 廖翊[1] 元艳宏[1] 闫荣[1] 阮长耿[2] 戴克胜[2] 

机构地区：[1]北京航空航天大学生物与医学工程学院血液与细胞生物学实验室,100191 [2]苏州大学第一附属医院、江苏省血液研究所 卫生部血栓与止血重点实验室

出　　处：《中华血液学杂志》2011年第9期618-621,共4页Chinese Journal of Hematology

基　　金：国家自然科学基金（30971067）;教育部博士研究生学术新人奖及北京航空航天大学博士创新基金（401059）

摘　　要：目的探讨血小板膜糖蛋白（GP）Ibα胞内区551到565氨基酸序列对GPIbα结合血管性血友病因子（VwF）功能的调控作用。方法以同时表达野生型GPIbα、GPIbβ和GPIX三种蛋白的中国仓鼠卵巢细胞株（1b9）、同时表达野生型GPIbβ、GPⅨ和在565或551以后氨基酸序列缺失的GPIbd的中国仓鼠卵巢细胞株（A565或A551）为模型，采用流式细胞术检测细胞株的GPIbα在瑞斯托霉素诱导下结合VwF的能力，流动腔技术检测细胞株在流体剪切力条件下（200s-1）在VWF表面的黏附状况，激光共聚焦技术检测细胞株在botrocetin诱导下在VwF表面的铺展状况。结果与对照lb9和A551相比，A565细胞株在ristocetin诱导下其GPIbα结合VwF的能力最强（P〈0．01），在VwF表面黏附的A565细胞数量最多（P〈0．05），在botrocetin诱导下A565细胞株在VwF表面的铺展面积最大（P〈0．05）。结论GPIbα胞内区551到565氨基酸序列对GPIbα结合vwF的功能具有重要调控作用。Objective To explore the role of the amino acids between 551 and 565 in the cytoplasmic domain of glycoprotein （GP） I bα in the VWF binding to GP I bα. Methods The VWF binding to GP I bα induced by ristocetin was analyzed by flow cytoinetry, in three GP I b-IX-expressing Chinese hamster ovary （CHO） cell lines Ib9, A565 and A551, adhesion of above cells on VWF by flow chamber analysis at shear rate of 200 s-l. The spread of GP I b-IX-expressing cells were stimulated with botrocetin on VWF-coated coverslips by confocal microscope. Results The VWF binding to GP I bα was higher in A565 cells stimulated by ristocetin than in △551 or 159 cells. The number of △565 cells adhered on the VWF-coated-chamber was more than that of controls at shear rate of 200 s-1. Moreover, the surface spreading areas of △565 cells were greater than that of the controls on VWF-coated coverslips. Conclusions The amino acids between 551 and 565 in the cytoplasmic domain of GP I bα regulates the VWF binding to GP I bα.

关 键 词：糖蛋白I bα 血管性血友病因子 

分 类 号：R363[医药卫生—病理学]