MicroRNA-134在缺氧预处理新生大鼠缺氧缺血脑组织中的表达及意义  

Expression of microRNA-134 in brain tissue of hypoxia-preconditioned neonatal rat hypoxic-ischemic models

作　　者：郑平 曾志涌 

机构地区：[1]深圳市龙岗区第二人民医院儿科,广东深圳518112

出　　处：《广州医学院学报》2011年第2期17-19,共3页Academic Journal of Guangzhou Medical College

基　　金：深圳市科技计划项目资助(201002159)

摘　　要：目的:观察micro RNA-134(miR-134)在缺氧预处理新生大鼠缺氧缺血后不同时期脑组织中的表达差异及其表达的特点及意义。方法:采用7日龄SD大鼠制备新生大鼠缺血缺氧性脑损伤动物模型。完全随机分为单纯缺血缺氧组、假手术对照组、缺氧预处理组(各18只)。3组又各分为处理后0h、1d、7d组(n=6)。每组6只用于荧光实时定量聚合酶链反应(qRT-PCR)观察miR-134转录量的差异。结果:miR-134在各组脑组织转录的表达量,单纯缺血缺氧组0 h(5.061±0.761)、1d(4.120±0.685)、7d(2.873±0.397);缺氧预处理组0 h(3.341±0.575)、1 d(2.769±0.351)、7d(1.658±0.290);假手术组0 h(6.617±1.988)、1d(5,798±1.116)、7d(5.984±1.879)。miR-134在单纯缺血缺氧组及缺氧预处理组脑组织的表达显著比假手术组减少(P<0.01),在同一时间段上,缺氧预处理组比单纯缺血缺氧组miR-134的表达明显减少(P<0.01)。同样在缺血缺氧后,无论是单纯缺血缺氧组还是缺氧处理组,miR-134的表达随着0h、1、7d的推移表达渐减少(P<0.05)。结论:miR-134的表达在调控神经系统发育中可能起着重要作用,缺氧预处理对新生大鼠缺血缺氧性脑损伤存在保护作用。Objective：To determine the expression of microRNA-134 （miR-134） in brain tissue of hypoxiapreconditioned neonatal rat models of hypoxia-ischemia at different days of life, and to identify the features and relevance of miR-134 expression in the pathogenesis of hypoxic ischemic encephalopathy （HIE）. Methods： Neonatal rat models of hypoxia-ischemia were established using 7-day-old SD rats. The rats were then randomized into several groups ： simple hypoxia-ischemic group（ HI group, n = 18 ）, sham-operated group（ SO group, n = 18 ） and hypoxia-preconditioned group（ HP group, n = 18 ）. Each of the groups was subdivided into Oh, ld and 7d groups（ n = 6 each）. The expression level of miR-134 in brain tissue was determined by qRT-PCR and compared among the groups. Results： The expression levels of brain miR-134 were 5. 061 ± 0. 761,4. 120 ± 0. 685 and 2. 873 ± 0. 397 in HI Oh, ld and 7d groups,3. 341 ± 0. 575,2. 769 ± 0. 351 and 1. 658 ± 0. 290 in the HP Oh, 1 d and 7d groups,and 6. 617 ± 1. 988,5. 798 ± 1. 116 and 5. 984 ± 1. 879 in the SO Oh, 1d and 7d groups, respectively. The level of brain miR-134 expression was significantly lower in HI and HP groups than in SO group （P 〈0.01 ）. At any given time spot,HP group had less expression of miR-134 than did HI group（P 〈0.01 ）. After exposure to hypoxia-ischemia, either HI or HP group showed decreasing level of miR-134 expression over time from 0h,ld to 7 d （P 〈 0.05 ）. Conclusion： Expression of miR-134 in brain tissues may be playing an important role in regulating the development of nervous system, and may also be protective against hypoxia- ischemic brain injury in hypoxia-preconditioned neonatal rats.

关 键 词：缺血缺氧性脑病 缺氧LIM—kinase 1(Limk1) microRNA-134(miR-134) 树突棘 

分 类 号：R741[医药卫生—神经病学与精神病学]