大鼠高架十字迷宫中的行为表现及海马GluR1蛋白水平的性别差异  

Sex difference in performance in elevated plus maze and hippocampal GluR1 level

作　　者：向小军[1] 郝伟[1] Therese A.Kosten 

机构地区：[1]中南大学湘雅二医院精神科,长沙410011 [2]美国德克萨斯州贝勒医学院门宁格精神病学和行为科学系,德克萨斯休斯顿77030

出　　处：《中南大学学报（医学版）》2011年第8期750-753,共4页Journal of Central South University ：Medical Science

基　　金：国家科技部"973"项目(2009CB522007);国家自然科学基金(30971050)~~

摘　　要：目的:探讨雌雄性SD大鼠在高架十字迷宫中各臂的探索时间及其海马内的GluR1蛋白水平是否存在性别差异。方法:将11只雄性和10只雌性SD大鼠放在高架十字迷宫中测试5min,30min后快速取出测试大鼠的两侧海马,在-80℃冰箱保存,然后提取蛋白质,用Western印迹方法检测海马内GluR1的蛋白水平。结果:与雄性大鼠相比,雌性大鼠在高架十字迷宫中表现出较轻的焦虑(P<0.05)。雌性大鼠海马总的GluR1水平及左侧海马GluR1水平比雄性大鼠低(P<0.05)。结论:SD大鼠在高架十字迷宫中的行为学表现以及海马内GluR1蛋白水平存在性别差异。Objective To explore whether sex difference exists in the performance in each arm of elevated plus maze （EPM） and GluR1 level in the hippocampus of female and male Sprague-Dawley rats.Methods Eleven male and 10 female SD rats were tested for 5 minutes in the EPM.These rats were decapitated 30 min after testing.The left and right hippocampus were dissected.Samples were stored at -80 ℃ for protein extracting.Western blot was used to detect the GluR1 levels in the hippocampus.Results Female rats exhibited less anxiety-like behaviors than male rats in the EPM （P〈0.05）.Female rats had lower GluR1 levels in total and left hippocampus than those of male rats （P〈0.05）.Conclusion Sex difference exists between female and male rats in the EPM and hippocampal GluR1.

关 键 词：GLUR1 焦虑 SD大鼠 性别 

分 类 号：R749.72[医药卫生—神经病学与精神病学]