华榛种仁油提取及GC-MS分析  

Extraction and GC-MS analysis of the oil from seed kernel of Corylus chinensis Franch

作　　者：刘祝祥[1] 陈功锡[1] 欧阳姝敏[1] 徐亮[1] 周建军[1] 

机构地区：[1]吉首大学植物资源保护与利用湖南省高校重点实验室,湖南吉首416000

出　　处：《中国油脂》2011年第9期14-17,共4页China Oils and Fats

基　　金：国家科技基础性工作专项重点项目(2008FY110400);湘西州科技创新专项(州科2006[045]号)

摘　　要：对采自湖南保靖白云山的华榛种仁,采用超声波提取法提取种仁油脂,设计正交实验比较不同超声强度、提取时间、溶剂种类、料液比对提取率的影响,获得最佳提取工艺条件为:以石油醚为溶剂,料液比1∶9,超声强度250 W,提取时间30 min,油脂提取率为57.84%,所得油脂橙黄清亮。利用气相色谱-质谱(GC-MS)联用技术对油脂脂肪酸成分及含量进行分析,得出油酸69.54%,亚油酸16.09%,9-十六碳烯酸0.22%,棕榈酸9.01%,硬脂酸4.44%,11-二十烯酸0.24%,花生酸0.23%,2-己基环丙烷辛酸0.14%,十七烷酸0.09%,其中不饱和脂肪酸含量高达86.09%,具有较高的应用价值。The seed kernels of Corylus chinensis Franch collected from Baiyun mountain in Baojing county of Hunan Province in China were assessed. Ultrasonic extraction method was used to extract the oil from seed kernel of Corylus chinensis Franch, and the effects of ultrasound intensity, extraction time, solvent sorts, solid - liquid ratio on extraction rate were studied by orthogonal test. The optimum conditions were determined as follows：petroleum ether used as extractant, extraction time 30 min, ul-trasound intensity 250 W, solid - liquid ratio 1 ： 9. Under the optimum conditions, the extraction rate was 57.84% , and the oil was orange and clear. Gas chromatography - mass spectrometry （ GC - MS） analysis showed that the fatty acid contents of the oil were： oleic acid 69.54% , linoleic acid 16.09% ,9 - hexadecenoic acid 0.22%, palmitic acid 9.01%, stearic acid 4.44%, 11 - eicosenoic acid 0.24%, eicosanoic acid 0.23% ,2 - hexyl - cyclopropaneoctanoic acid 0.14%, and heptadecanoic acid 0.09%. Among them,the unsaturated fatty acid content was as high as 86.09%.

关 键 词：华榛 种仁油 超声波提取 GC—MS 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程]