纯钛表面氧化钛和氧化锆涂层的结构性能及细胞毒性  

Microstructure properties and cytotoxicity of titania and zirconia coating on titanium surface

作　　者：司家文[1] 万浩元[1] 胡启凡[2] 孙惠强[1] 

机构地区：[1]山东大学口腔医学院山东省口腔生物医学重点实验室 [2]山东大学医学院临床医学系,济南250012

出　　处：《国际口腔医学杂志》2011年第5期531-534,538,共5页International Journal of Stomatology

基　　金：山东省自然科学基金资助项目(ZR2010HM035);2008年度国家大学生创新性实验计划基金资助项目(081042224)

摘　　要：目的研究纯钛表面氧化钛/氧化锆复合涂层的显微结构及其对金瓷结合强度的影响,评价其细胞毒性。方法将柱形纯钛试件随机分为试验组和对照组,以等离子喷涂技术在试验组试件表面制备氧化钛/氧化锆复合涂层,运用能量散射光谱、扫描电子显微镜等技术观察其显微结构。将两组试件与Titankeramik瓷粉烧结形成金瓷复合体,测定其金瓷结合强度。制备涂层浸提液,以甲噻唑四唑氮比色评价其细胞毒性。结果氧化钛/氧化锆复合涂层无明显裂隙,金瓷界面结合紧密。试验组试件的金瓷结合强度高于对照组(P<0.05)。试验组试件涂层的细胞毒性为0～1级,各试验组及阴性对照组细胞增殖曲线几乎重叠,且呈线性增加趋势。结论氧化钛/氧化锆复合涂层与基体结合良好,可以显著地增强纯钛金瓷的结合强度,而且具有人体应用的生物安全基础。Objective To investigate the microstructure and properties of titania（TiO2） and zirconia（ZrO2） coated on titanium and to test its vitro cytotoxicity.Methods The titanium specimens were divided into test group and control group,and test group was coated with a TiO2/ZrO2 complex film on the surface by plasma spraying technology.The microstructure of the coating was analyzed by energy-dispersive spectrometry and scanning electron microscope.The metal-porcelain bond samples were made from both groups and Titankeramik porcelain which were used for metal-porcelain bond strength measurement.The extraction liquid of the coating was obtained and cell relative growth rate（RGR） was calculated through methyl thiazolyl tetrazolium assay by which the cytotoxicity was graded.Results The TiO2/ZrO2 coating were well bonded with titanium and no obvious fracture was observed.The metal-porcelain bonding strength of test group were higher than control group significantly（P〈0.05）.The toxicity gradation of all groups was 0-1,RGR of all groups were almost the same and increased with culture time.Conclusion The results indicate that the TiO2/ZrO2 coating are well bonded with titanium which can improve the metal-porcelain bond strength of titanium and is compatible with the tested cells.

关 键 词：纯钛 等离子喷涂 涂层 结合强度 细胞毒性 

分 类 号：R783.1[医药卫生—口腔医学]