光动力学疗法诱导卵巢癌细胞系Skov3和宫颈癌细胞系Hela凋亡的体外研究  

Photodynamic therapy-induced the apoptosis of ovarian and cervical cancer cell lines in vitro studies

作　　者：毛滢[1] 沈铿[1] 魏薇[1] 

机构地区：[1]卫生部北京医院妇产科,100730

出　　处：《中华老年医学杂志》2011年第9期779-783,共5页Chinese Journal of Geriatrics

摘　　要：目的研究光动力疗法对人宫颈癌细胞系Hela及卵巢癌细胞系Skov3的体外增殖及凋亡的影响，探讨光动力学在老年女性妇科肿瘤治疗的优势。方法采用不同能量激光对不同浓度光敏剂（5-ALA）处理过的宫颈癌细胞系（Hela）及卵巢癌细胞系（Skov3）进行照射，48h后应用光镜、电镜进行形态学观察，并采用四甲基偶氮唑蓝（MTT）比色法检测光动力学治疗对肿瘤细胞体外生长的抑制作用，采用流式细胞仪检测细胞凋亡情况。结果实验组细胞经光动力学处理后体积缩小，细胞变形、皱缩，细胞核浓缩、深染，染色质密集成斑块状，细胞间失去彼此连接，透射电镜下可见致密的染色质沿核膜下聚集，有凋亡小体形成。0．1、0．5、1．0、2．5mmol／L的5-ALA处理过的肿瘤细胞经不同能量0．1、0．5、2．5、12．5J／cm。激光照射后细胞系Skov3和Hela的抑制率分别为12．9％～90．7％和3．9％～70．0％；光敏剂浓度、光照强度对卵巢癌细胞系Skov3与宫颈癌细胞系Hela吸光度值（A）均有影响（P〈O．01），光敏剂与光照间交互作用显著（P〈0．01）。随光敏剂浓度与光照强度的增加两细胞系A值呈降低趋势，呈光敏剂浓度与光照时间的依赖性（P〈0．01）。结论光动力学治疗对体外生长的卵巢癌细胞系Skov3及宫颈癌细胞系Hela有抑制作用。Objective The apoptosis and anti-proliferation effects of photodynamic methods on cervical cancer and ovarian cancer cell lines were studied to explore the advantage of photodynamic therapy in gynecological cancer. Methods Cervical cancer cell line （Hela） and ovarian cancer cell line （SKOV3） were treated with photosensitizer （5-ALA） at different doses and then radiated by laser with energy scopes. The morphological characteristics were observed by light microscopy and electronic microscopy. Inhibition effects and ceil apoptosis were studied by MTT assay and flow cytometry （FCM） Results The cytomorphosis and shrinkage, nucleus condensation and anachromasia, dense chromatin lining along cell membrane were observed in both cell line receiving photodynamic therapy. Through transmission electron microscope （TEM）, we found nucleus chromatin gathering along membrane of cell nuclear and apoptotie body formed. The cell inhibition rates were 12.9%-90.7%and 3.9 %-70.0 %, respectively in Skov3 cell and HeLa cell treated with 5-ALA of different concentrations （0.1, 0.5, 1.0, 2.5 mol/L） and receiving laser of different doses （0.1, 0.5, 2.5, 12.5 J/cm2）. The concentration of the photosensitizer and the dose of laser influenced the optical density（A） of cell lines （P〈0.01）. The photo sensitizer was related strongly to laser （P〈0.01）. The optical density of the two cell lines decreased with the increases of photo sensitizer concentration and laser dose （P〈0.01）, showing concentration- and time-dependent manner. Conclusions Photodynamic therapy inhibits the growth of ovarian cancer and cervical cancer cell line.

关 键 词：光化学疗法 细胞凋亡 卵巢肿瘤 宫颈肿瘤 

分 类 号：R737.31[医药卫生—肿瘤]