FTIR-HATR诊断α-地中海贫血  

Identification of α-Thalassemia by FTIR-HATR Spectroscopy

作　　者：郑元涛[1] 彭立新[2] 姚辉璐 黄庶识[3] 王桂文[3] 

机构地区：[1]广西科学院生物研究所,广西南宁530007 [2]广西科学院非粮生物质酶解国家重点实验室国家非粮生物质能源工程技术研究中心广西生物炼制重点实验室,广西南宁530007 [3]广西科学院生物物理实验室,广西南宁530007

出　　处：《广西科学》2011年第3期228-232,共5页Guangxi Sciences

基　　金：国家自然科学基金项目(No.60868002);广西科学基金项目(No.0832022Z)资助

摘　　要：将傅里叶红外光谱与水平衰减全反射技术(FTIR-HATR)结合,对α-地中海贫血样品与正常样品进行检验,通过改进的相对强度计算方法进行对比分析。结果表明:α-地贫组与正常组间在I1074/I1152、I1517/I1074、I3295/I3372相对强度上存在显著差异;在峰的指认上,波数1074cm-1、1152cm-1、1517cm-1和3295cm-1分别表征磷酸类化合物、碳水化合物、酪氨酸和Amide A,其相对强度差异应主要来源于细胞内增加的高磷化合物2,3-二磷酸甘油酸、减少的葡萄糖以及蛋白组分;由于以上组分的变化被相对强度计算所放大,在组间呈显著的特异性,通过相对强度与主成分析均能将混合的样品正确区分。FTIR associated with multi-bounce horizontal attenuated total reflectance was used to diagnose α-thalassemia patients.In ranges of 800~1780cm^-1 and 2480~3600cm^-1,we calculated all intensity ratios to detect spectra differences between patient and health groups.We found that there are significant differences between α-thalassemia patients and health groups in intensity ratios of I1074/I1152,I1517/I1074 and I3295/I3372.At the peaks of 1074cm^-1,1152cm^-1,1517cm^-1 and 3295cm^-1,they are assigned to deformation vibration of O-P=O,C-O stretching vibration of carbohydrate,breathing vibration of tyrosine ring and Amide A,respectively.It suggests that the difference of intensity ratios between two groups may result from increasing 2,3-diphosphoglycerate,decreasing glucose and hemoglobin in α-thalassemia patients.Furthermore,principal components analysis（PCA） was used to assess these ratios and demonstrated a correct classification on the basis of PC scores.

关 键 词：血红蛋白 Α-地中海贫血 FTIR-HATR 相对强度 

分 类 号：O657.33[理学—分析化学] R556[理学—化学]