HPLC-ELSD法测定大蜜丸中蜂蜜的糖成分含量  

Measurement on Sugar Composition of Honey In Dami Pill with HPLC-ELSD

作　　者：李晗[1] 李荣 杨瑞瑞 齐星[1] 

机构地区：[1]陕西中医学院,2009级研究生陕西咸阳712046 [2]陕西省食品药品检验所,陕西西安710061

出　　处：《陕西中医学院学报》2011年第5期81-83,共3页Journal of Shaanxi College of Traditional Chinese Medicine

摘　　要：目的建立高效液相-蒸发光散射检测法同时测定蜂蜜中果糖、葡萄糖、蔗糖的方法。方法 AgiLent Car-bohydrate色谱柱,乙腈-水(75:25)为流动相,流速2.0 mL/min,激光蒸发光散射检测器,漂移管温度90℃。结果葡萄糖、果糖、蔗糖的线性范围分别为0.484～14.532μg/mg,0.469～14.088μg/mg0,.486～14.580μg/mg,平均回收率103.95%,102.90%,97.56%,RSD 3.37%,4.82%,2.01%。结论本方法可有效地控制大蜜丸中蜂蜜的质量。Objective To establish HPLC - ELSD to measure the composition of fructose, glucose and sucrose in honey. Methods AgiLent Carbohydrate Chromatographic Column, Acetonitrile -water （75：25 ） in mobile phase with speed of 2.0mL/min, Evaporative Light - Scattering Detector and drift tube with temperature of 90℃ have been applied in the experiment. Results Linear range of fructose, glucose and sucrose expands from 0. 484 -14. 532μg/mg, 0. 469 -14. 088μg/mg,0.486 -14.580μg/mg respectively, with average recovery of 103.95%, 102.90% ,97.56%, RSD 3.37%, 4.82% and 2.01%. Conclusion This method may effectively control both the quantity and quality of honey in Dami Pill.

关 键 词：大蜜丸 蜂蜜 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]