ITO微孔电化学传感器的构建及细胞胞吐的监测  

Fabrication of ITO Micro-Pore Electrochemical Sensor and the Detection of Exocytosis

作　　者：赵辉[1] 李玲[1] 何品刚[1] 方禹之[1] 

机构地区：[1]华东师范大学化学系,上海200062

出　　处：《电化学》2011年第3期278-282,共5页Journal of Electrochemistry

基　　金：国家自然科学基金(No.20575022)资助

摘　　要：胞吐是细胞之间传递信号的主要方式之一,安培法监测细胞胞吐是研究细胞间通讯的基本方法.本研究采用ITO(Indium Tin Oxide)导电玻璃与紫外光刻技术构建了一种新型ITO微孔电化学传感器,用于细胞胞吐现象的监测并取得较好的效果.相比传统方法,ITO微孔电化学传感器不仅可以取代传统的碳纤维微电极,而且可与荧光或化学发光等方法联用,运用多种手段同时对细胞的生理现象进行监测.Exocytosis is one of the major means for cells to transmit messages.Amperometric detection of exocytosis is an important method in studying communication between cells.In this study a new type of ITO micro-pore electrochemical sensor was fabricated by means of ITO and UV light lithographic mask and was used in the detection of exocytosis with promising results.Compared with the conventional method,the ITO micro-pore sensor not only can replace the carbon fiber electrode,but also can combine with fluorescent or chemiluminescent methods in detecting the physiological phenomena of cells.

关 键 词：胞吐 SH-SY5Y 单细胞分析 ITO 

分 类 号：Q26[生物学—细胞生物学]