花生种子脂肪酶活力定量测定及其与储藏特性的相关性  

Quantitative Determination of Peanut Seed Lipase Activity and Its Correlation with Storage Characteristics

作　　者：高奇[1,2] 张瑛[1,3] 刘泽[1] 张正竹[2] 吴跃进[3] 禹山林[4] 张琛[2] 滕斌[1] 吴敬德[1] 蒋岚 

机构地区：[1]安徽省农业科学院水稻研究所/安徽省水稻遗传育种重点试验室,安徽合肥230031 [2]安徽农业大学农业部茶及药用植物安全生产重点开放试验室,安徽合肥230036 [3]中国科学院安徽省离子束生物工程学重点试验室,安徽合肥230031 [4]山东省花生研究所,山东青岛266100

出　　处：《作物学报》2011年第9期1676-1682,共7页Acta Agronomica Sinica

基　　金：安徽省自然科学基金资助项目(11040606M97);安徽省优秀青年基金项目(08040106802);安徽省水稻遗传育种重点试验室开放基金项目(SD2009-4)资助

摘　　要：对已有水稻脂肪酶活力比色测定方法进一步修改和优化,建立了花生脂肪酶活力定量测定方法,并确定取样量5mg、底物浓度30mgmL-1为最适试验条件,该方法具有精确性好、特异性强、重复性好等特点。利用该方法检测216份花生种质资源的脂肪酶活性,表明不同材料间差异极显著(P<0.001)。试验筛选出酶活力高低差异显著的材料10份(高低各5份),并以其中2份高脂肪酶活性和2份低脂肪酶活性材料进行高温、高湿人工加速老化试验,结果表明,随老化时间延长,高脂肪酶活性材料发芽率下降较快,而低脂肪酶活性材料发芽率下降较慢,花生脂肪酶活力与其种子活力呈负相关(相关系数为-0.8875)。该结果为进一步深入研究脂肪酶对花生储藏劣变的影响提供了参考。Based on colorimetric determination method reported for rice lipase,we established a new method improving and optimizing the measurement for peanut seed lipase activity,with the optimum sampling amount of 5 mg and substrate concentration of 30 mg mL^-1.The method was validated to be accurate,specific and well repeatable.The lipase activities of 216 peanut germplasm resources were detected,with significant difference（P〈0.001） among them.Five with higher lipase activity and five with lower lipase activity were screened,and each two of which were selected to perform an accelerated-aging experiment under high temperature and high moisture.The results indicated that,with extension of storage time,the germination rate of peanut with lower lipase activity decreased slowly,while that of peanut with higher lipase decreased rapidly,showing a negative correlation between seed vigor and peanut lipase activity（correlation coefficient was-0.8875）.The study provides a reference for the further research in the effect of lipase on peanut storage characteristics.

关 键 词：花生 脂肪酶活力 定量测定 储藏特性 

分 类 号：S565.2[农业科学—作物学]