脑缺血生化机制的研究——脑缺血CaM含量和分布及亚细胞结构的变化  

STUDIES ON BIOCHEMICAL MECHANISM OF BRAIN ISCHEMIA (Ⅱ) CHANGES OF CaM CONTENTS, DISTRIBUTION AND INFRASTRUCTURE IN BRAIN ISCHEMIA

作　　者：唐放鸣[1] 张光毅[1] 赵文君[1] 赵昇皓 董红燕[2] 陈世超[2] 

机构地区：[1]徐州医学院生物化学教研室 [2]徐州医学院电子显微镜室

出　　处：《徐州医学院学报》1990年第2期118-122,共5页Acta Academiae Medicinae Xuzhou

摘　　要：用ELISA和免疫胶体金标记电镜技术,研究了蒙古沙土鼠急性脑缺血CaM的含量、分布和亚细胞形态结构变化。ELISA测定结果;缺血10分钟,CaM下降25％;缺血30分钟,CaM下降45％。免疫胶体金检查:正常组每个视野胶体金颗粒数为9.95±3.76,缺血组为5.32±2.44,两组相比,P值<0.001。电镜下可见缺血神经元粗面内质网脱颗粒,线粒体基质密度增加等改变。The changes ofCaM contents , distribution and ultrastructure in acute brain ischemia were investigated by immunogold staining (IGS) and enzyme-linked immuno-sorbent assay (ELISA). The ELISA resulls showed that the contents of CaM decreased by 25 and 45% at 10 and 30 min after ischemia, respectively. The numbers of immunogold particle in each field of vision decreased significantly from 9.95 + 3.76 to 5.32+2.44 after 30 min of isch-emia(p<0.001).After 30 min of ischemia, the degranulation of the RER and the increase of the matrix density in the Mit were observed by electron mic roscopy.

关 键 词：脑缺血 钙调素 生化机制 细胞结构 

分 类 号：R743.310.2[医药卫生—神经病学与精神病学]