前房相关免疫偏离中调节性T细胞的变化  

Variation of regulatory T cell during anterior chamber-associated immune deviation

作　　者：张敏敏[1] 张晓敏[1] 赵少贞[1] 

机构地区：[1]天津医科大学眼科中心屈光角膜科,天津300384

出　　处：《天津医科大学学报》2011年第3期322-325,共4页Journal of Tianjin Medical University

基　　金：天津市科委留学基金绿色通道资助项目(07JCBJC16500)

摘　　要：目的:探索前房相关免疫偏离(ACAID)机制中调节性T细胞的变化,为进一步研究其具体机制创造条件。方法:将C57BL/6小鼠分为ACAID实验组、阳性对照组和正常组。实验组将2μL卵白蛋白(OVA,50 mg/mL)前房注射,阳性对照组和正常组以2μL PBS行前房注射,7 d后实验组和阳性对照组足底皮下免疫OVA100μL(2.5 mg/mL OVA与CFA等体积充分乳化),正常对照组不予处理。14 d后检测耳廓迟发型超敏反应(DTH),流式分析脾细胞中CD4+CD25+Foxp3+T细胞以及CD8+Foxp3+T细胞的数量和比例。结果:DTH结果显示,实验组的耳廓肿胀值较阳性对照组明显减小。CD4+CD25+Foxp3+T细胞和CD8+Foxp3+T细胞比例较阳性对照组和正常组均升高。结论:CD4+CD25+Foxp3+T细胞和CD8+Foxp3+T细胞在ACAID机制中发挥重要作用。Objective： To examine the variation of regulatory T cell during anterior chamber-associated immune deviation （ACAID）, and determine the mechanism of ACAID. Methods： C57BL/6 mice were divided into 3 groups, ACAID group, positive control group and normal group. 2 μL OVA （50 mg/mL） were injected into the anterior chamber of ACAID group. Normal group and positive control group received AC injection of PBS. 7 d later, all the mice received subcutaneous injections of 100 μL OVA （ 2.5 mg/mL OVA emulsified h l in complete Freuned adjuvant）. 14 d later, DTH response was measured to evaluate the development of ACAID. Flow cytometry was used to assay the frequency of CD4^＋CD25^＋Foxp3^＋T cell and CD8^＋Foxp3^＋T cell in the spleen of C57BL/6. Results： A significantly decreased DTH response was observed in the ACAID group, a high level of CD4^＋CD25^＋Foxp3^＋T cell and CD8^＋Foxp3^＋T cell were observed in ACAID group ,compared with normal group and positive control group. Conclusion： CD4^＋CD25^＋Foxp3~＋T cell and CD8^＋Foxp3^＋T cell play important roll in the mechanism of ACAID.

关 键 词：前房相关免疫偏离 调节性T细胞 FOXP3 小鼠 

分 类 号：R77[医药卫生—眼科] R392.1[医药卫生—临床医学]