槲皮万寿菊素金属配合物的合成、抗氧化活性及与DNA结合的研究  

Synthesis,antioxidative activity and DNA-binging of metalliccomplexes of quercetagetin

作　　者：张秉君[1] 王萃娟[1] 邱光美[1] 黄帅[1] 王友松[1] 周先礼[1] 

机构地区：[1]西南交通大学生命科学与工程学院,四川成都610031

出　　处：《化学研究与应用》2011年第9期1152-1158,共7页Chemical Research and Application

基　　金：新世纪优秀人才基金(NECT-08-0820);西北大学合成与天然功能分子化学教育部重点实验室开放基金;西南交通大学科技发展基金;中央高校基本科研业务费专项资助(SWJTU09ZT29;SWJTU2010ZT09)项目

摘　　要：合成了槲皮万寿菊素铜(Ⅱ)、锌(Ⅱ)铁(Ш)三种配合物,采用红外、紫外光谱及元素分析等方法分析了其配位情况,研究了配合物清除DPPH自由基、超氧阴离子自由基和羟基自由基的活性,并运用紫外光谱滴定实验,研究了配体及配合物与ct-DNA的相互作用。结果表明,配合物较配体具有更强的清除DPPH自由基、超氧阴离子自由基和羟基自由基活性;槲皮万寿菊素及其配合物以插入模式与DNA结合,与DNA结合作用的大小顺序为:Que-Fe>Que-Cu>Que-Zn>Que。Three kinds of Cu（II）,Zn（II）,Fe（Ш） complexes of quercetagetin were synthesized and characterized by IR spectra,UV-spectra and elemental analysis.The free radical（DPPH·,O2ˉ·,·OH） scavenging activities of the complexes were investigated.The mechanism of the interaction between the free ligand and complexes with calf thymus DNA（ct-DNA） were investigated by means of UV spectra.The results showed that free radical scavenging activities of the complexes on DPPH·,O2ˉ·and·OH were obviously stronger than that of the quercetagetin.Results of spectroscopic measurements suggested that the three complexes bound to DNA via an intercalative mode and calculated DNA-binding action decreased in the order Que-FeQue-CuQue-Zn Que.The new metallic complexes of quercetagetin added new contents for coordination chemistry.The good bioactivity of the complexes afford an applied foundation on the medicine.

关 键 词：槲皮万寿菊素金属配合物 光谱表征 自由基 抗氧化活性 DNA作用 

分 类 号：O627[理学—有机化学]