拮抗菌A02代谢产物对几种果树病害的活性  

The Antifungal Activity of the Metabolite of Biocontrol Strain A02 Against Some Fruit Tree Diseases

作　　者：刘霆[1] 陶万强 王合 花玉鹏[1,3] 刘伟成[1] 

机构地区：[1]北京市农林科学院植物保护环境保护研究所,北京100097 [2]北京市林业保护站,北京100029 [3]河南农业大学植物保护学院,河南郑州450002

出　　处：《北方园艺》2011年第17期13-16,共4页Northern Horticulture

基　　金：北京市自然科学基金资助项目(6101001);北京市科技计划资助项目(D0705002040291);北京市农林科学院资助项目(2010A006)

摘　　要：以北京市农林科学院植物保护保环境保护研究所自主分离筛选的利迪链霉菌A02为供试菌株,采用离体生物测定法评价了其对葡萄灰霉病菌(Botrytis cinerea)、桃褐腐病菌(Monilinia fructicola)和枣青霉病菌(Penicilliumsp.)3种果树病原真菌的室内毒力,通过小区试验测定了其对葡萄霜霉病的田间防效。结果表明:A02活性产物对葡萄灰霉病菌、桃褐腐病菌和枣青霉病菌均具有强毒力,对其菌丝生长的抑制中浓度分别为1.3696、8.1115和7.4989mg/L,最低抑菌浓度均在30mg/L以下;对葡萄霜霉病的田间小区防效达95.7%,显著高于对照生物农药10%宝丽安可湿性粉剂和1%多抗霉素水剂。Streptomyces lydicus A02,which isolated from Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences.Evaluated pathogen fungi indoor toxic against Botrytis cinerea,Monilinia fructicola and Penicillium sp.By the strains bioassays evaluation method and the field plot experiment respectively.The results showed that the metabolite of strain A02 presented a strong toxicity against Botrytis cinerea,Monilinia fructicola and Penicillium sp.with the 50% inhibiting concentration（IC50） of 1.3696,8.1115 and 7.4989 mg/L respectively and the minimal inhibitory concentration（MIC） lower than 30 mg/L.Its control efficacy against grape downy mildew in field plot was up to 95.7%,which was significantly（0.05） higher than that of the two control agents,10% WP and 1% AS of Polyoxin.In conclusion,the antifungal metabolite of S.lydicus A02 may be used as a safe and effective agent to control fruit tree diseases.

关 键 词：利迪链霉菌 纳他霉素 果树病害 室内毒力 田间防效 

分 类 号：S436.6[农业科学—农业昆虫与害虫防治]