IP_3 Sensitive Calcium Channel Involved in the Jasmonic Acid Induced Calcium Mobilization  

IP_3敏感的钙离子通透性通道参与茉莉酸诱导的钙动员(英文)

作　　者：李洋洋[1] 于涌鲲[1] 万善霞[1] 赵福宽[1] 孙清鹏[1] 于同泉[2] 潘金豹[2] 

机构地区：[1]北京农学院生物技术学院,北京102206 [2]北京农学院植物科学技术学院,北京102206

出　　处：《Agricultural Science & Technology》2011年第9期1265-1269,共5页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(30700428,30911130166);Natural Science Foundation of Beijing Municipality(5072009);The New Star Plan of Science and Technology in Beijing Municipality(2006B26)~~

摘　　要：[Objective] This study was to investigate the role of IP3 sensitive calcium channel in the JA-induced calcium mobilization pathway.[Method] Arabidopsis thaliana leaves were labeled by Fluorescent probe Fluo-3/AM under low temperature at 4 ℃ to measure the fluorescent intensity of intracellular Ca2＋ which was pretreated with heparin on jasmonic acid（JA）-induced.[Results] When A.thaliana leaf cells were pretreated with 10,50 or 100 ng/ml heparin,intercellular free Ca2＋ fluorescence intensity was reduced in comparison with negative control.Once the heparin-pretreated A.thaliana leaf cells were stimulated with 100 μmol/L JA,intercellular Ca2＋ fluorescence intensity increased gradually and tended to be stable at a degree equivalent with that in negative control.[Conclusion] The experiment showed that the pretreatment with heparin could inhibit the increase of the intracellular Ca2＋ concentration significantly which JA-induced in leaves of Arabidopsis thaliana.[目的]研究茉莉酸诱导的钙动员通往IP3敏感的钙离子通透性通道的作用。[方法]以低温导入法将钙离子荧光探针Fluo-3/AM导入拟南芥叶片细胞中,利用LASAF(Leica Application Suite-Advanced Fluorescence)软件记录肝素对茉莉酸(JA)诱导的胞内钙离子荧光强度的变化。[结果]经不同浓度的肝素预处理后,拟南芥叶细胞中胞内钙离子的荧光强度降低,再用100μmol/LJA处理时,其荧光强度升高,但仅与未经肝素处理的荧光强度相当。[结论]肝素预处理可抑制JA诱导的胞内钙离子浓度的升高。

关 键 词：Arabidopsis thaliana Ca2＋ HEPARIN Jasmonic acid 

分 类 号：Q946[生物学—植物学]