Effects of Glycosyltransferase Gene sm-Ngt1 on the Plant Height of Rice  

糖基转移酶基因sm-Ngt1对水稻株高的影响(英文)

作　　者：王巍葳[1] 谭艳平[1] 王春台[1] 刘学群[1] 

机构地区：[1]中南民族大学生命科学学院/生物技术国家民委重点实验室,湖北武汉430074

出　　处：《Agricultural Science & Technology》2011年第9期1279-1282,共4页农业科学与技术（英文版）

基　　金：Supported by National Science Foundation of China(30871318);Key Research Project of Ministry of Education,PRC(210265)~~

摘　　要：[Objective] The aim was to study the effects of over-expressed sm-Ngt1,a glycosyltransferase gene induced by both methyl jasmonate and salicylic acid from tobacco,on the plant height of rice.[Method] The binary expression vector of the sm-Ngt1 gene was constructed and transferred to matured embryo of indica rice YTB with the method of Agrobacterium infection.The height of the positive transgenic plants was measured.[Result] 117 positive transgenic plants with sm-Ngt1 were obtained.The results showed that the rice plants dwarfed with different degrees after transferring the sm-Ngt1 gene,the height of 37% of transgenic plants is 71.4±9.8 cm,27% is 65.1±4.6 cm,and the average height of YTB（CK） is 130.0±4.3 cm.[Conclusion] These results aid a foundafion for further study on function of sm-Ngt1.[目的]研究烟草中受甲基茉莉酸和水杨酸双重诱导的糖基转移酶基因sm-Ngt1过表达对水稻生长的影响。[方法]构建sm-Ngt1的植物表达载体,采用农杆菌浸染法转化籼稻粤泰B,对获得的阳性转基因植株进行高度检测。[结果]共获得117株阳性转sm-Ngt1植株。在成功转入sm-Ngt1基因后,水稻植株出现了不同程度的矮化,其中37%的转基因植株高度为71.4±9.8cm,27%的转基因植株高度为65.1±4.6cm,而未转基因的对照YTB的平均高度为130.0±4.3cm。[结论]为进一步研究sm-Ngt1的功能奠定了基础。

关 键 词：GLYCOSYLTRANSFERASE sm-Ngtl RICE DWARF 

分 类 号：S511[农业科学—作物学]