Study on the Inhibitory Effect of Sophora japonica Extracts against the Growth of Microcystis aeruginosa  

龙爪槐提取物对铜绿微囊藻生长的抑制作用研究(英文)

作　　者：周晓见[1,2] 夏洁[1] 靳翠丽[1,2] 缪莉[1,2] 董昆明[1,2] 封克[1,2] 

机构地区：[1]扬州大学环境科学与工程学院,江苏扬州225127 [2]扬州大学海洋科学与技术研究所,江苏扬州225127

出　　处：《Agricultural Science & Technology》2011年第9期1347-1349,1354,共4页农业科学与技术（英文版）

基　　金：Supported by National Natural Science Foundation of China(41076097,41006097);Science and Technology Research Key Project of ChineseMinistry of Education(211065);Natural Science Foundation of Jiangsu Province,China(BK2010322);Open Research of Jiangsu Key Laboratory of Environmental Material and Environmental Engineering(K090027,K090025,K090026,K090028);Graduate Science and Technology Innovation Project of Department of Education,Jiangsu Province,China(M080960);"New Century"Talent Project of Yangzhou University,China~~

摘　　要：[Objective] The research aimed to discuss the inhibitory effect of Sophora japonica extracts against the growth of Microcystis aeruginosa.[Method] The inhibitory effect of extracted liquid of Sophora japonica leaf against the growth of M.aeruginosa was measured.Moreover,the active component was studied and analyzed initially.[Result] The absolute alcohol extract of Sophora japonica leaf was separated by n-hexane,ethyl acetate,n-butanol and water phases in turn.The polar fractions were found being the majority （〉60%）.The non-polar fraction in n-nexane （about 25%） was found significantly inhibiting the growth of M.aeruginosa.The inhibition rates of fraction in n-hexane at the concentrations of 25 and 50 mg/L against M.aeruginosa in 7 d were higher than 75% and 90% respectively.In addition,chlorophyll a of M.aeruginosa was also destroyed in the presence of the hexane fraction.[Conclusion] The research provided the theoretical basis for preventing and controlling the water bloom of M.aeruginosa.[目的]探讨龙爪槐提取物对铜绿微囊藻生长的抑制作用。[方法]测定了龙爪槐叶片浸取液对铜绿微囊藻的增殖抑制效果,并对活性组分进行了初步研究和分析。[结果]龙爪槐叶片的无水乙醇浸提物经正己烷、乙酸乙酯、正丁醇和水依次分配后,提取物主要成分为极性组分(60%以上),而含量约为25%的非极性组分(正己烷相)具有显著的抑制铜绿微囊藻生长的效果。在25和50mg/L正己烷相组分作用下铜绿微囊藻的7d生长抑制率分别达75%和90%以上,同时还伴随着细胞中叶绿素a的破坏。[结论]为防治微囊藻水华提供了理论依据。

关 键 词：Sophora japonica Microcystis aeruginosa N-hexane phase Chlorophyll a 

分 类 号：X173[环境科学与工程—环境科学]