木糖发酵高产2,3-丁二醇菌株的选育  

Breeding of Klebsiella pneumoniae Strains with High-Production of 2,3-Butanediol from Xylose

作　　者：张翠英[1] 肖冬光[1] 韩宁宁[1] 郭学武[1] 

机构地区：[1]工业微生物教育部重点实验室天津市工业微生物重点实验室天津科技大学生物工程学院,天津300457

出　　处：《食品研究与开发》2011年第9期168-170,共3页Food Research and Development

基　　金：国家"863计划"(2006AA020101);天津科技大学科学研究基金项目(20090401)

摘　　要：以肺炎克雷伯氏菌(Klebsiella pneumoniae)CICC10011为出发菌株,进行紫外诱变选育。通过2,3-丁二醇抗性和产酸能力对诱变菌株进行初筛,发酵后23,-丁二醇产量检测进行复筛,获得6株高产2,3-丁二醇的肺炎克雷伯氏菌,其中产量最高的一株诱变菌U3-17,与出发菌株相比,产量提高了21.5%。在摇瓶批式补料发酵中,诱变株U3-17的23,-丁二醇产量达49.3 g/L。Klebsiella pneumoniae CICC10011 was used as parent strain and mutagenized by ultraviolet radiation.Though the fast screening method based on acid production and 2,3-butanediol tolerance,six mutants from Klebsiella pneumoniae 10011 with high-yielding 2,3-butanediol were obtained.Among them,the yeild of 2,3-butanediol by the best excellent mutant U3-17 fermentation in shake-flask was 21.5 % higher than that by the parent strain fermentation.The fed-batched cultivations with xylose were studied,and concentration of 2,3-butanediol by U3-17 fermentation in shake-flask reached 49.3 g/L.

关 键 词：肺炎克雷伯氏菌 2 3-丁二醇 木糖发酵 诱变育种 

分 类 号：TQ923[轻工技术与工程—发酵工程]