紫外诱变结合化学诱变选育凝乳酶高产菌的研究  

Screening of Bacillus Subtilis JAASB Strains With High-yield Chymosin by UV and Chemicals Mutation

作　　者：高岩[1] 王景会[1,2] 李玉秋[2] 马颖辉[3] 杨贞耐[2] 

机构地区：[1]吉林农业大学食品科学与工程学院,长春130118 [2]吉林省农业科学院农产品加工中心,长春130033 [3]黑龙江八一农垦大学,大庆163319

出　　处：《农产食品科技》2011年第3期28-31,共4页

基　　金：现代农业产业技术体系建设专项资金资助（CARS-37）.

摘　　要：采用紫外诱变和硫酸二乙酯（DES）诱变剂对产凝乳酶的枯草芽孢杆菌JAASB进行诱变选育，在酪蛋白平板上以凝乳圈直径与菌落直径的比值为指标进行初筛，在液态发酵培养基上进行复筛，选育到三株具有遗传稳定性的高产菌株JAASB－3、JAASB－5、JAASB－14，其凝乳活力分别为1142．6Su，mL、1263．2SU／mL、1333．3SU／mL，比出发菌株分别提高了52％、68％、78％。The starting strain Bacillus subtilis JAASB producing ehymosin was mutated by UV and DES. Effect of mutation was determined by preliminary screening based on the ratio between milk-clotting zone and colony diameter, and secondary screening based on liquid fermentation. Three mutant strains with good genetic stability and high chymosin activity were obtained. The chymosin activity produced by JAASB-3 ,JAASB-5 and JAASB-14 was 1142.6S U/mL,1263.2 SU/mL,1333.3 SU/mL, respectively.Comparing with the starting strain, chymosin activity produced by JAASB-3 ,JAASB-5 and JAASB-14 was increased by 52%,68% and 78%, respectively.

关 键 词：凝乳酶 紫外诱变 化学诱变 枯草芽孢杆菌 

分 类 号：TS201.25[轻工技术与工程—食品科学]