DAPK基因启动子区过甲基化与颈段食管癌的关系  

Hypermethylation of Death-associated Protein Kinase Promoter in neck epipharynx squamous cell cancer

作　　者：吴凯[1] 李欣[1] 马茂强[1] 张勇[1] 

机构地区：[1]山东中医药大学附属医院,250000

出　　处：《中国医学创新》2011年第27期3-5,共3页Medical Innovation of China

摘　　要：目的探讨死亡相关蛋白激酶基因启动子区过甲基化与颈段食管癌的关系。方法采用甲基化特异性PCR(MSP)法和逆转录聚合酶链反应(RT-PCR)法分析颈段食管部正常黏膜、颈段食管癌及相应癌旁组织中DAPK基因启动子区过甲基化及其mRNA表达状况。结果 42例颈段食管癌组织中,有27例(64.3%)DAPK基因启动子区过甲基化,其在各病理分级和T分级之间差异无统计学意义(P>0.05),而在N分级之间差异有统计学意义(P<0.05)。25例癌旁组织中,有6例过甲基化,且均为颈段食管癌组织中有过甲基化的病例。颈段食管部正常组织、非甲基化的颈段食管癌组织和癌旁组织中均有DAPK mRNA表达。结论颈段食管癌中DAPK基因启动子区过甲基化与mRNA失表达有关,可能是颈段食管癌发生、发展的原因之一。Objective To explore the relationship between hypermethylation of the promoter of death - associated protein kinase （DAPK）gene and neck epipharynx squamous cell cancer. Methods Promoter hypermethylation and mRNA expression of DAPK gene were detected by methylation - specific PCR, RT - PCR and gene sequencing. Results Among the 42 patients with neck epipharynx squamous cell cancer, hypermethylation of DAPK ptomoter was detected in 27 cases （64.3 % ）. There was no significant difference in hypermethylation in relation to pathological grade and clinical staging （P 〉 0. 05 ）, but a highly significant difference was observed between patients with and without lymphnode metastasis（ P 〈 0. 05 ）. DAPK mRNA was not expressed in normal neck epipharynx mucosa, neck epipharynx squamous cell cancers without hypermethylation and tumor adjacent tissues. Conclusion Hypermethylation of DAPK promoter is associated with loss of its transcription in neck epipharynx cell carcinoma. The high frequency hypermethylation of DAPK promoter illustrates its potential clinical application as tumor marker for diagnosis and prognosis.

关 键 词：颈段食管肿瘤 蛋白激酶类/遗传学 基因表达 DNA甲基化 

分 类 号：R735.1[医药卫生—肿瘤]