高效液相色谱测定蕨类植物中氨基酸含量  

Determination of Amino Acid Content in Five Species of Fern by HPLC with Precolumn Derivation

作　　者：刘恒霞[1] 余鹏[1] 王慧忠[1] 

机构地区：[1]滁州学院化学与生命科学系,安徽滁州239000

出　　处：《氨基酸和生物资源》2011年第3期30-33,共4页Amino Acids & Biotic Resources

基　　金：滁州学院校级重点自然项目(2010kj005z);滁州学院大学生科研项目(2010xs054)

摘　　要：以5种蕨类植物(大羽贯众、美丽复叶耳蕨、黄山鳞毛蕨、狗脊蕨和紫萁)根状茎为研究材料,利用邻苯二甲醛柱前衍生高效液相色谱法分析了材料中的氨基酸成分。鉴于材料中蛋白质含量较低,测定方法中增加了样品的使用量并采用了梯度洗脱程序。结果表明,测试的5种蕨类植物所含总氨基酸含量较低,很少有超过5%,尤其在狗脊蕨中只有1.74%。在所测定的17种氨基酸中,5种蕨类植物所含氨基酸含量之间的差异并不是非常显著,只有蛋氨酸含量差异较大。其中,紫萁根状茎中蛋氨酸含量最高,达到0.035%,美丽复叶耳蕨中蛋氨酸含量未被检出。基于柱前衍生的高效液相色谱法同样适合测定蕨类植物样品中的氨基酸含量。The amino acid content in Cytomium macrophyllum Tagawa,Dryopteris hwangshanensis Ching,Woodwardia japonica（L.f.）Sm.,Arachniodes amoena（Ching）Ching and Osmunda japonica Thunb.was determined by HPLC with precolumn derivation.As these samples contained low protein,the determination could be done by increased the biological sample,and by HPLC with gradient elution.The results showed that the samples of 5 tested fern species all contained low amino acids,and the amino acid content in Arachniodes amoena was only 1.74%.There was a little difference of content of the amino acids in these ferns,but methionine.For example,there was 0.035%,the highest content of methionine in Osmunda japonica but few in Dryopteris hwangshanensis Ching.The HPLC with precolumn derivation could be used to determine amino acid content in fern samples.

关 键 词：蕨类植物 根状茎 氨基酸 柱前衍生 高效液相色谱法 

分 类 号：Q946[生物学—植物学]