阿托伐他汀对糖尿病ApoE基因敲除小鼠组织中CD55和CD59表达的影响  

Effects of atorvastatin on complement regulatory proteins CD55 and CD59 expressions on aortas in streptozotocin-induced diabetic ApoE-knock out mice

作　　者：马西文[1] 秦明照[2] 赵焕英[3] 常志文[2] 张勇[2] 宋爱丽[3] 

机构地区：[1]河南大学附属郑州市第一人民医院内分泌老年病科,郑州450004 [2]首都医科大学附属北京同仁医院,北京100730 [3]首都医科大学,北京100069

出　　处：《中国实用神经疾病杂志》2011年第17期1-4,共4页Chinese Journal of Practical Nervous Diseases

基　　金：郑州市科技发展计划项目(编号:112PPTSF306-2);郑州市医学博士创业基金;河南省卫生科技创新型人才工程专项经费资助;郑州市创新型科技人才队伍建设工程项目

摘　　要：目的观察阿托伐他汀对糖尿病ApoE基因敲除小鼠主动脉组织中CD55、CD59表达的影响。方法 22只雄性6周龄ApoE基因敲除小鼠随机分为糖尿病动脉粥样硬化(对照)组(12只)和阿托伐他汀干预(干预)组(10只),予链脲佐菌素(55 mg.kg-1.d-1体质量)连续5次腹腔注射建立糖尿病模型,普通饲料喂养。造模成功后,干预组予阿托伐他汀(10 mg.kg-1.d-1体质量)灌胃8周。监测生化指标,测量斑块面积;采用免疫荧光和Real-time PCR技术,观察阿托伐他汀干预后小鼠主动脉组织中CD55和CD59的表达变化。结果与对照组相比,阿托伐他汀干预后体质量增加,血糖和血脂无变化,血清淀粉样蛋白-A[(5.9±3.2)μg/mL vs.(3.8±1.6)μg/mL,P>0.05]和动脉粥样硬化病变面积呈减少趋势[(264046.4±88374.97)μm2vs.(218752.4±87927.16)μm2,P>0.05]。免疫荧光显示,阿托伐他汀干预后小鼠主动脉组织中CD55和CD59表达较对照组增强。Real-time PCR结果显示,与对照组比较,阿托伐他汀干预后CD55和CD59 mRNA相对表达量无明显变化(0.55±0.2 vs.0.43±0.11,0.76±0.36 vs.0.55±0.13,P均>0.05)。结论独立于调脂作用之外,阿托伐他汀可能具有上调CD55和CD59蛋白表达的作用。Objective To investigate the effects of atorvastatin on CD55 and CD59 expressions on aortas in streptozotocin-induced diabetic ApoE-knock out mice.Methods At 6 weeks of age,twenty-two male ApoE-knock out mice were obtained and divided into two groups： diabetic atherosclerotic group（n=12）,and atorvastatin group（n=10）.Mice were rendered diabetic by intraperitoneal injection of streptozotocin 55 mg/kg of body weight/day for 5 consecutive days.After 8 weeks of treatment with atorvastatin 10 mg/kg of body weight/day,venous bloods were collected for glucose and lipids analysis,and aorta were prepared for atherosclerotic plaques,immunofluorescence and Real-time PCR.Results There were no differences in glucose and lipids between diabetic atherosclerotic group and atorvastatin group.After treatment with atorvastatin,there was a trend towards decrease in serum amyloid-A[（5.9±3.2）μg/mL vs.（3.8±1.6）μg/mL,P0.05）]and the atherosclerotic plaques[（218752.4±87927.16）μm2 vs.（264046.4±88374.97）μm2,P0.05）].Immunofluorescence showed that CD55 and CD59 protein expressions increased in atorvastatin group compared with diabetic atherosclerotic group.Real-time PCR showed that levels of CD55 and CD59 mRNA were similar in diabetic atherosclerotic group and in atorvastatin group（0.55±0.2 vs.0.43±0.11,0.76±0.36 vs.0.55±0.13,P0.05）.Conclusion Independent of the effects on lipids,atorvastatin could increase CD55 and CD59 protein expressions in diabetic ApoE-knock out mice.

关 键 词：糖尿病 动脉粥样硬化 CD55 CD59 阿托伐他汀 ApoE 

分 类 号：R-332[医药卫生]