生长抑素及其功能性受体表达与胰腺腺泡细胞外分泌的关系  

Study of the relationship among somatostatin,its receptor subtypes and the external secretion of pancreatic acinar cells

作　　者：蔡佳[1] 黄文祥[1] 

机构地区：[1]重庆医科大学附属第一医院感染科,400016

出　　处：《重庆医学》2011年第27期2714-2716,2718,共4页Chongqing medicine

摘　　要：目的探讨SD大鼠胰腺腺泡细胞上的生长抑素受体(SSTR)各亚型的分布及生长抑素具体与哪种亚型的SSTR结合发挥作用。方法急性分离大鼠胰腺腺泡细胞,取得胰腺腺泡细胞悬液。用Trizol法提取各时间段胰腺腺泡细胞的mRNA并在PCR扩增仪上得到产物,使用Labimage图像分析系统对电泳结果进行量化分析。结果 SSTR1～5在SD大鼠胰腺腺泡细胞上有表达,其表达分别为0.980±0.085;0.766±0.064;0.850±0.078;0;2.010±0.225。生长抑素作用15、30 min后SSTR1～5的表达分别为0.908±0.059;1.142±0.078;0.798±0.030;0;1.852±0.132和0.891±0.049;1.347±0.063;0.764±0.071;0;1.791±0.102。SSTR2表达较对照组明显增高(P<0.05)。结论 SD大鼠胰腺腺泡细胞上存在SSTR1～3、5亚型。生长抑素与SSTR2受体结合后,发挥其抑制胰腺腺泡细胞外分泌的作用。Objective To detect the expression of somatostatin receptor（SSTR） mRNA of all five subtypes on SD rat pancreatic acini and identify the functional somatostatin subtype receptor which will couples to somatostatin.Methods Isolated pancreatic acini were separated acutely from rat pacrea.The total mRNA of all tubes were extracted and the expression of SSTR1-5 mRNA were detected by RT-PCR.Finally the electrophoresis results were analyzed by the image analysis system-Labimage.Results The results showed that there was SSTR1-5 in the pancreatic acinar cells of rats,the expression was 0.980±0.085,0.766±0.064,0.850±0.078,0 and 2.010±0.225 respectively.And then the expression of SSTR1-5 changed to 0.908±0.059,1.142±0.078,0.798±0.030,0 and 1.852±0.132;0.891±0.049,1.347±0.063,0.764±0.071,0 and 1.791±0.102 when acini were treated with somatostatin for 15 min.Among them,SSTR2 was significantly increased compared with control group（P0.05）.Conclusion Four subtypes of SSTR（SSTR1-3,5）express in pancreatic acini.We suppose that somatostatin regulates the secretion of pancreatic acini and Iks when it is coupled to SSTR2.

关 键 词：生长抑素受体 胰腺腺泡细胞 功能性受体 

分 类 号：R576[医药卫生—消化系统]