P16基因启动子在脑胶质瘤患者中的异常甲基化及意义  

THE SIGNIFICANCE OF P16 GENE PROMOTER HYPERMETHYLATION IN PATIENTS WITH GLIOMAS

作　　者：朱沛传 陈江瑛[2] 张素平[2] 

机构地区：[1]广州市从化温泉镇医院,广东广州510976 [2]暨南大学附属第四医院广州市红十字会医院,广东广州510220

出　　处：《现代医院》2011年第9期9-12,共4页Modern Hospitals

基　　金：广东省医学科研基金(编号:A2010466);广东省中医药局科研立项(编号:A2010020);广州市医药卫生科技一般引导项目立项项目(编号:2009-YB-042);广州市中医药;中西医结合科研课题(编号:2009A25)

摘　　要：目的探讨脑胶质瘤患者肿瘤与血浆标本P16基因启动子区CpG岛的甲基化状态及其与临床的关系,分析脑胶质瘤的可能发生机制及早期诊断方法。方法用甲基化特异性PCR对40例脑胶质瘤患者肿瘤及血浆标本P16基因启动子区进行甲基化检测。结果 40例脑胶质瘤标本的P16基因启动子区异常甲基化率为35%(14/40),相应血浆标本的P16的异常甲基化检出率为25%(10/40),两者相比差异无显著性(p>0.05)。20例对照组脑组织标本的P16基因甲基化率为5%(1/20),与胶质瘤标本比较差异具有统计学意义(p<0.05)。P16基因启动子区的异常甲基化与患者年龄、性别、肿瘤病理类型、恶性程度的差异无统计学意义(p>0.05)。结论 P16基因启动子区CpG岛的异常甲基化在脑胶质瘤的发生中起着重要作用,检测血浆标本的P16的异常甲基化对于脑胶质瘤早期诊断具有重要意义。Objective To investigate the methylation status of the CpG islands in the promoter regions of P16 gene in cancer tissue and peripheral plasma of patients with gliomas and to analyze its clinical significance,discussing the possible mechanism and early diagnosis technique of gliomas. Methods We analyzed the hypermethylation status of P16 gene in 40 glioma tissues and corresponding blood plasma of glioma patients by methylation specific-PCR. Results The frequency of methylation of Promoter of RASSF1A gene was 35%（14/40）in glioma tissue,25%（10/40）in peripheral blood plasma,there was no significant difference（p0.05）.Frequency of methylation was 5%（1/20） in normal brain tissue,which has significant difference from the glioma tissue.no significant association of abnormal methylation was also identified with the patient＇s gender,age,pathological type,pathological stage（p0.05）. Conclusion Hypermethylation of CPG islands in the promoter of P16 gene in gliomas may contribute to the development of gliomas.Detection of hypermethylation of P16 in blood plasma of glioma patients is valuable in early diagnosis of gliomas.

关 键 词：胶质瘤 P16基因 甲基化 甲基化特异性PCR 

分 类 号：R735.9[医药卫生—肿瘤]