一株南极生境来源木霉菌株抗菌及诱导植物抗盐抗寒作用的初步研究  

PRELIMINARY STUDY OF ANTIBACTERIAL ACTIVITY OF A TRICHODERMA STRAIN FROM AN ANTARCTIC MOSS HABITAT,AND ITS ABILITY TO PROMOTE SALT AND COLD RESISTANCE IN PLANTS

作　　者：贾炜[1,2] 田黎[1,2] 陶晶晶[1] 史振平[1] 张久明[1] 

机构地区：[1]青岛科技大学,山东青岛266042 [2]国家海洋局第一海洋研究所,山东青岛266061

出　　处：《极地研究》2011年第3期189-195,共7页Chinese Journal of Polar Research

基　　金：国家高技术研究发展计划(2011AA10A202;2011AA09070402);国家科技支撑计划(2011BAE06804;青岛科技计划基础研究项目(10-3-4-4-7-jch);国家自然科学基金(40776098;40976104)资助

摘　　要：由南极苔藓样品中分离到一株木霉菌株NTYM-0112,利用传统分类学与ITS序列及系统发育分析相结合方法对其进行分类鉴定,并对其抑植物病原菌活性、范围及对植物抗盐、抗寒的诱导作用进行了初步研究。结果表明,该菌株属于棘孢木霉(Trichoderma perellum),对稻瘟霉菌(Pyricularia oryzae)、棉花黄萎菌(Verticillium dahliae)、小麦赤霉病菌(Fusarium graminearum)等7种常见植物病原菌有明显的抑制作用。番茄种子经该菌株处理后发芽率比对照提高了13.04%,番茄苗盐害指数降低了35.7%,成活率提高了34.4%,干重含水量提高了20.4%,叶片内丙二醛含量比对照明显减少;黄瓜幼苗经该菌株处理后,在0℃、5℃、10℃下电解质外渗率比对照分别下降了13.1%、20.9%、32.8%,可溶性糖外渗值分别下降了18.0 mg/g、28.6 mg/g、48.4 mg/g,证明该菌株对植物具有良好的抑菌和诱导抗逆作用。Using traditional taxonomic methods, ITS sequences and phylogenetic analysis, Trichoderma strain NTYM- 0112 isolated from an Antarctic moss habitat was identified as Trichoderma asperellum. A preliminary study was made of its antibacterial activities and range in inducing salt and cold resistance effects in plants. The antibacterial activities results revealed that strain NTYM-0112 showed obvious inhibition to seven common plant pathogens inclu- ding Pyricularia oryzae, VerticiUium dahlia and Fusarium graminearum. Salt resistance results indicated that the germination rate of tomato seeds increased 13.4% compared with the control after treatment with strain NTYM- 0112 ; the salt injury index reduced significantly by 35.7% ; and the survival rate and water content of tomato dry weight increased by 34.4% and 20.4% , respectively, after treatment with strain NTYM-0112. The MDA content of leaves was obviously reduced compared with the control. The cold resistance results showed that after treatment with strain NTYM-0112, the electrolyte leakage rate of cucumber seedlings at 0℃, 5℃ and 10℃ reduced by 13. 20.9% and 32.8%, respectively, and the extravasation values of soluble sugar decreased by 18.0 mg/g, 28.6 mg/g and 48.4 mg/g, respectively. All of these results confirmed Antarctic habitat strain Trichoderma asperellum possessed preferable antibacterial activity and effect of inducing plant resistance to stress.

关 键 词：南极生境木霉 分离 鉴定 抑菌活性 抗盐 抗寒 

分 类 号：Q93[生物学—微生物学] S332[农业科学—作物遗传育种]