NMDA受体2A亚单位mRNA和蛋白质在大鼠海马前脑缺血再灌注损伤中的变化  

The changes of mRNA and protein of NMDA receptor subunit 2A in rat medels with hippocampus forebrain ischemia-reperfusion injury

作　　者：张旭[1] 滕大才[2] 徐铁军[2] 高伟[1] 

机构地区：[1]北京大学首钢医院神经内二科,北京市100144 [2]徐州医学院解剖学和神经生物学教研室

出　　处：《河北医药》2011年第19期2894-2896,共3页Hebei Medical Journal

摘　　要：目的观察分析大鼠前脑缺血再灌注早期,NMDA受体2A亚单位mRNA和蛋白质表达的改变,探讨两者在缺血性脑损伤中的变化和相互关系。方法选择SD大鼠30只,随机为分正常对照组(NC组)6只,假手术对照组(SC组)12只,缺血再藻注组(IR组)12只,大鼠脑8μm厚石蜡切片,原位杂交染色,TUNEL染色,焦油紫染色。图像和镜下分析。结果 (1)NR2AmRNA在海马的CA1区缺血24h后,显著高于NC组水平,是SC组的141.5%(P<0.01)。(2)NR2A蛋白质水平在海马的CA1区缺血复灌48h后,显著下降,是SC组的59.3%(P<0.01)。结论缺血性脑损伤早期中,NMDA受体2A亚单位mRNA和蛋白质表达并不呈现一致变化,提示缺血过程中2A亚单位的转录水平未受影响,而翻译水平受到了干扰。Objective To observe the changes of expression of mRNA and protein of NMDA receptor subunit 2A in rat medels with hippocampus forebrain ischemia-reperfusion （I/R） injury, and to explore the correlation between the expression of mRNA and that of protein. Methods 30 SD rats were randomly divided into three group, normal control group （ NC group, n = 6）, sham - operation control group （ SC group, n = 12 ）, ischemia-reperfusion group （IR group, n = 12）. The paraffin section （thickness：8μm） was performed in brain tissues of rats, then in situ hybridization staining, TUNEL staining and cresyl violet staining were carried out, finaly,the examination results were analysed under microscope. Results The levels of NR2A mRNA in CA1 region of hippocampus 24 hours after ischemia were significantly higher than those in NC group, which were as high as 141.5% of SC group （ P 〈0.01 ）. The protein levels of NR2A were obviously decreased 48 hours after I/R, which was as low as 59.3% of SC group （ P 〈 0.01 ）. Conclusion At the early period of ischemic brain injury, the expression betweem mRNA and protein of NMDA receptor subunit 2A is not accordant, which suggests that during the ischemia,the transcriptional levels of 2A subunit are not affected, however, its translational levels are affected.

关 键 词：NMDA受体2A亚单位 脑缺血 免疫组织化学 原位杂交 海马 大鼠 

分 类 号：R743.31[医药卫生—神经病学与精神病学]