扶正活血不同组方对肝纤维化大鼠TGFβ1和TIMP-1表达的影响  

Impacts of Fuzheng Huoxue Formulas on TGFβ1 and TIMP-1 Expressions in the Rats with Liver Fibrosis

作　　者：刘中景[1] 王者令 罗玮敏[1] 宋霆[1] 孙晓慧[1] 柳盛[1] 

机构地区：[1]青岛市传染病医院,山东青岛266033

出　　处：《世界中西医结合杂志》2011年第9期760-762,共3页World Journal of Integrated Traditional and Western Medicine

基　　金：青岛市中医药科研计划重点资助(No.2009-ZYZ002)

摘　　要：目的观察扶正活血不同组方对肝纤维化大鼠肝血清及肝组织中血清转化生长因子β1(TGFβ1)和基质金属蛋白酶抑制剂-1(TIMP-1)表达水平的影响,探讨其抗肝纤维化的作用机理及疗效差异。方法将90只SD大鼠随机分为六组,采用CCl4加橄榄油造模,治疗组分为扶正活血1号方、2号方、3号方,并设阳性药物组、模型组和正常对照组,通过对各组动物灌服不同药物及生理盐水,分别检测大鼠血清和肝组织中TGFβ1、TIMP-1的表达水平。结果扶正活血不同组方对大鼠肝组织与血清中TGFβ1和TIMP-1表达水平明显低于模型组(P<0.05或P<0.01)和阳性对照组(P<0.05或P<0.01),而1号方和2号方的改善作用较3号方明显。结论扶正活血不同组方均能有效抑制TGFβ1、TIMP-1的表达,从而减少或降低肝纤维化的发生。Objective To observe the impacts of fuzheng huoxue formulas on the expressions of transforming growth factor β1（TGFβ1）and inhibitor of matrix metalloproteinase-1（TIMP-1）in liver serum and liver tissue of the rats with liver fibrosis and explore the mechanism on anti-liver fibrosis and the efficacy difference.Methods 90 SD rats were divided randomly into six groups.The models were set up with CC14 and olive oil.The treatment groups were subdivided into a fuzheng huoxue No.1 formula group,No.2 formula group and No.3 formula group.Additionally,a positive medicinal group,a model group and a normal control group were designed.The rats in each group were treated with gastric infusion of different medicines and physical saline.The expressions of TGFβ1 and TIMP-1 were detected in the serum and liver tissue of rats.Results The expressions of TGFβ1 and TIMP-1 in liver tissue and serum of rats in each formula group were lower remarkably than those in either model group（P0.05 or P0.01）or positive control group（P0.05 or P0.01）.The improvements in No.1 and No.2 formula groups were apparent than those in No.3 formula group.Conclusion Fuzheng huoxue formulas effectively inhibit the expressions of TGFβ1 and TIMP-1 and reduce the occurrence of liver fibrosis.

关 键 词：扶正活血组方 血清转化生长因子β1(TGFβ1) 基质金属蛋白酶抑制剂-1(TIMP-1) 肝纤维化大鼠 

分 类 号：R575.2[医药卫生—消化系统]