小鼠骨髓移植修复卵巢功能损伤  

Therapeutic effects of bone marrow transplantation on ovarian injury in mice

作　　者：梁利霞[1] 晁岚[1] 邓晓惠[1] 于红玲[1] 刘文军[2] 王旭平[3] 

机构地区：[1]山东大学齐鲁医院不孕不育诊疗中心,山东济南250012 [2]山东大学齐鲁医院病理科,山东济南250012 [3]山东大学齐鲁医院心血管重构与功能研究重点实验室,山东济南250012

出　　处：《南方医科大学学报》2011年第9期1534-1538,共5页Journal of Southern Medical University

基　　金：山东省科技攻关项目(2010DGSF10814)

摘　　要：目的研究骨髓移植对化疗引起的小鼠卵巢功能损伤的修复作用。方法 48只小鼠随机分为3组:正常对照组(A组)、化疗并骨髓移植组(B组)和化疗组(C组)。B、C组小鼠每天给予环磷酰胺150 mg/kg腹腔注射,连续3 d。1周后,对B组小鼠进行骨髓移植。给药起第21、50天分两批处死小鼠,称量卵巢、子宫湿重,观察卵巢组织学变化。用Hoechest33342荧光染料标记骨髓细胞,移植入预先注射环磷酰胺的小鼠体内,2 d后取小鼠卵巢做冰冻病理切片,荧光显微镜下观察。结果给药第21、50天,B组小鼠的卵巢系数、各级卵泡数目明显高于C组而低于A组,均具有统计学意义(P<0.05)。受体鼠的卵巢内发现显示荧光的供体鼠骨髓细胞。结论骨髓移植对化疗药物引起的卵巢功能损伤具有修复作用。Objective To evaluate the therapeutic effects of bone marrow transplantation（BMT） on ovarian injury induced by chemotherapy in mice.Methods Forty-eight mice were randomized equally into normal control group（A）,cyclophosphamide and BMT group（B）,and cyclophosphamide group（C）.The mice in groups B and C were treated with intraperitoneal injection of cyclophosphamide at the daily dose of 150 mg/kg for 3 consecutive days,and allogeneic bone marrow cell transplantation was performed in group B.The ovary coefficient and the amount of follicles were compared to evaluate the function of ovaries.For cell tracking,the bone marrow cells were labeled with Hoechest 33342 and detected through fluorescence microscope after transplantation.Results On days 21 and 50 after cyclophosphamide treatment,the ovary coefficient and the amount of follicles were significantly lowered in groups B and C（P〈0.05）,but the reduction was obviously ameliorated in group B（P〈0.05）.Cell tracking showed the presence of the donor bone marrow cells in the ovaries of the recipients mice after BMT.Conclusion BMT can improve the ovarian function impaired by chemotherapy in mice.

关 键 词：骨髓移植 环磷酰胺 卵巢 小鼠 

分 类 号：R730.5[医药卫生—肿瘤]