意向运动疗法对大鼠局灶性脑缺血后GFAP和SYP表达的影响  

Effect of willed movement therapy on GFAP and SYP expression in rats with cerebral ischemia-reperfusion

作　　者：侯德仁[1] 沙地克.沙吾提 刘建锋[1] 朱海霞[1] 邓锦凤[1] 胡中扬[1] 周军[3] 刘远新[2] 

机构地区：[1]中南大学湘雅三医院神经内科,湖南长沙410013 [2]新疆医科大学附属中医医院脑病一科,新疆乌鲁木齐830000 [3]中南大学湘雅三医院中心实验室,湖南长沙410013

出　　处：《南方医科大学学报》2011年第9期1543-1546,共4页Journal of Southern Medical University

基　　金：湖南省科技厅项目(2010SK3110;2011FJ3206)

摘　　要：目的探讨意向运动疗法对大鼠局灶性脑缺血2 h后再灌注缺血周围脑组织GFAP和SYP表达的影响及其对神经再生修复作用的可能机制。方法采用线栓法建立(middle cerebral artery occlusion,MCAO)大鼠模型,缺血2 h后进行再灌注;再灌注24 h后根据Longa神经功能缺损评分选择实验模型,随机分为模型组(MCAO组)、环境改变组(environmental modification,EM)、意向运动组(willed movement,WM),每组再分为3、7、15 d三个亚组;大鼠处死前再进行Longa评分;通过免疫组织化学及免疫荧光观察缺血周围脑组织GFAP和SYP的表达。结果再灌注15 d时,WM组大鼠神经功能缺损评分低于MCAO组、EM组(P<0.05);再灌注7、15 d时,WM组大鼠GFAP和SYP的表达量均高于MCAO组、EM组(P<0.05);MCAO组与EM组比较:任一时间点的GFAP和SYP表达均无统计学差异(P>0.05)。结论意向运动疗法促进了MCAO大鼠神经功能缺损的恢复;可能与其促进缺血周围脑组织GFAP和SYP的表达有关。Objective To determine the effect of willed movement on the expression of glial fibrillary acidic protein（GFAP） and synaptophysin（SYP） in adult rats with cerebral ischemia-reperfusion,and explore the mechanism of willed movement in promoting nerve repair and regeneration.Methods Adult rat models of cerebral ischemia-reperfusion injury were established by middle cerebral artery occlusion（MCAO） for 2 h followed by a 24-h reperfusion.The models were then divided randomly into 3 groups,namely the model group,environmental modification（EM） group,and willed movement（WM） group.In each group,neurological deficits were evaluated at 3,7 and 15 days after reperfusion.Immunohistochemistry and immunofluorescence assay were employed to examine the expression of GFAP and SYP in the brain tissue near the ischemic foci.Results The rats in WM group showed lessened neurological deficits at 15 days and lowered expression of GFAP and SYP at 7 and 15 days after reperfusion compared with the model and EM groups（P〈0.05）.No significant difference was found in the expression of GFAP or SYP between the model group and EM group at any time points.Conclusion Willed movement can promote the functional recovery of neurological deficits following cerebral ischemia-reperfusion probably in relation to enhanced GFAP and SYP expressions in the ischemic brain tissues.

关 键 词：意向运动 脑缺血 胶质纤维酸性蛋白 突触素 

分 类 号：R741[医药卫生—神经病学与精神病学]