大肠癌患者VEGF和Endostatin的表达及其与临床病理特征的关系  

Serum levels of vascular endothelial growth factor and endostatin expression in colorectal carcinoma patients and the relationships with clinical and pathological characteristics

作　　者：刘福国[1] 冀玉苓[2] 潘新亭[3] 路艳艳[1] 孙学国[1] 高艳景[4] 陈建[4] 江月萍[1] 

机构地区：[1]青岛大学医学院附属医院消化内科,山东青岛266003 [2]青岛大学医学院附属医院信息科,山东青岛266003 [3]青岛大学医学院附属医院普外科,山东青岛266003 [4]山东大学齐鲁医院消化内科,山东济南250012

出　　处：《中国现代普通外科进展》2011年第8期602-605,共4页Chinese Journal of Current Advances in General Surgery

基　　金：山东省卫生厅科技资助项目(2001CAICJB9)

摘　　要：目的:探讨大肠癌患者手术前后血清血管内皮生长因子(VEGF)和Endostatin的动态变化规律及其与临床病理特征的关系。方法:ELISA法检测大肠癌患者(大肠癌组)术前及术后2周血清VEGF和Endostatin水平,并与大肠腺瘤患者(大肠腺瘤组)和健康对照者(对照组)进行比较。结果:1)大肠癌组术前血清VEGF水平显著高于大肠腺瘤组及对照组(P均<0.01)。2)大肠癌组术前血清Endostatin水平显著高于大肠腺瘤组及对照组(P均<0.01)。3)大肠癌组术前血清VEGF、Endostatin水平与原发肿瘤大小、细胞分化程度、区域淋巴结转移、肝转移及Dukes分期密切相关(P均<0.05),与性别、肿瘤部位等因素无关(P>0.05)。4)大肠癌组术后2周血清VEGF水平较术前显著下降,而血清Endostatin水平较术前升高(P均<0.01)。结论:大肠癌患者血清VEGF和Endostatin水平升高,且与原发肿瘤大小、细胞分化程度、区域淋巴结转移、肝脏转移及Dukes分期等因素有密切关系;血清VEGF和Endostatin水平是评价大肠癌恶性行为、预测浸润和转移程度的有效指标。Objective： To study the relationship between the serum levels of vascular endothelial growth factor（VEGF）,endostatin and clinical and pathological characteristics in colorectal carcinoma patients.Methods： Serum VEGF and endostatin levels were measured by enzyme-linked immunoassay in 58 patients with colorectal carcinoma before and after surgical resection（two weeks later） and in control groups including 30 patients with adenoma of colon and 30 healthy persons.Results： 1）The preoperative serum level of VEGF in patients with colorectal carcinoma（279.28±81.67 pg/mL） was significantly higher than that in patients with adenoma of colon（126.60±23.81 pg/mL） and healthy persons（117.54±25.27 pg/mL）.2）The preoperative serum level of endostatin in patients with colorectal carcinoma（59.48±18.79 ng/mL） was significantly higher than that in patients with adenoma of colon（35.19±6.35 ng/mL） and healthy persons（32.82±6.71 ng/mL）.3）The preoperative serum levels of VEGF and endostatin in patients with colorectal carcinoma were closely related to the size of the primary tumors,grade of cell differentiation,regional lymph-node metastasis,liver metastasis and Dukes＇ stage,but their relations to tumor site and sex were not significant.4）The postoperative serum level of VEGF in patients with colorectal carcinoma（175.40±58.23 pg/mL） was decreased significantly than that of pre-operation,while postoperative serum endostatin level（71.33±17.39 ng/mL） was increased significantly than that of pre-operation.Conclusions： Elevated serum VEGF and endostatin levels in patients with colorectal carcinoma are closely correlated to the size of the primary tumors,grade of cell differentiation,Dukes＇ stage and metastasis.Serum VEGF and endostatin assay may be used to preoperatively evaluate the biological behavior and predict invasion and metastasis of colorectal carcinoma.

关 键 词：大肠肿瘤 血管内皮生长因子 ENDOSTATIN 酶联免疫吸附试验 

分 类 号：R735.3[医药卫生—肿瘤]