半乳凝素1在口腔黏膜上皮癌变过程中的表达及意义  

Expression of galectin-1 in carcinogenesis of oral mucosal epithelium

作　　者：魏魁杰[1] 潘红芽[1] 杨筱[1] 钟来平[1] 叶冬霞[1] 张志愿[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院口腔医学院口腔颌面外科上海市口腔医学研究所上海市口腔医学重点实验室,200011

出　　处：《中华口腔医学杂志》2011年第9期524-527,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金（30630065）;浙江省自然科学基金（572091171）

摘　　要：目的探讨半乳凝素1（galectin-1）在口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）中的表达及临床意义，旨作为OSCC早期诊断的检测指标。方法分别采用实时定量聚合酶链反应（PCR）、蛋白质印迹法和免疫组化方法检测口腔黏膜上皮体外癌变模型、OSCC细胞系及30例原发OSCC标本中的半乳凝素1mRNA及蛋白质的表达。结果半乳凝素1在永生化细胞系（human immortalized oral epithelia cell，HIOEC）中mRNA和蛋白相对表达量平均为0，071±0.023和0.118±0.046，与永生化细胞系HIOEC相比，半乳凝素1在所有检测的体外癌变模型和OSCC细胞系中mRNA和蛋白相对表达水平均升高，分别为0．141±0．049和0．504±0．33（P〈0．01）。30例OSCC组织中半乳凝素lmRNA和蛋白相对表达量（0．059±0．034、1．5±0．68）均显著高于癌旁组织（0．029±0．012、0．4±0．56）（P〈0．01）。结论半乳凝素1在151腔黏膜上皮癌变过程中表达上调可能与OSCC的发生、发展有关，可作为OSCC早期诊断的检测指标。Objective To investigate the expression of galectin-1 in oral squamous cell carcinoma （OSCC） and its clinical significance. Methods Detection of the mRNA and protein expression of galectin-I in the in vitro cellular carcinogenesis model of OSCC, OSCC cell lines and tissue specimens from 30 primary OSCC patients were performed using real-time polymerase chain reaction （PCR）, Western blotting and immunohistochemistry, respectively. Results The value of galectin-1 mRNA and protein level in human immortalized oral epithelia cell （ HIOEC ） cell was 0. 071 ±0. 023,0. 118 ± 0. 046, Compared with the HIOEC, galectin-1 mRNA level and protein expression were increased significantly in all the cell lines （0. 141±0. 049,0. 504 ±0. 33 ） （P 〈 0. 01 ）. The levels of mRNA and protein expression of galectin-1 were significantly higher in the cancerous tissue（0. 059±0. 034,1.5±0. 68） than in the normal adjacent tissues （0. 029±0. 012,0. 4 ±0. 56 ） （P 〈 0. 01 ）. Conclusions The expression of galectin-1 gene up-regulated in carcinogenesis process of OSCC significantly may be related to the tumorigenesis and development of OSCC, which illustrates its potential clinical application as tumor marker for early diagnosis.

关 键 词：癌 鳞状细胞 半乳凝素1 早期诊断 

分 类 号：R739.8[医药卫生—肿瘤]