真养产碱杆菌H16合成聚-β-羟基丁酸酯的培养基优化  

Optimization of the Composition for Poly-β-hydroxybutyrate Production by Ralstonia eutropha H16

作　　者：许懿[1] 陈荣辉[1] 赖瑞华[1] 王远鹏[1] 李清彪[1,2] 何宁[1] 

机构地区：[1]厦门大学化学化工学院 [2]厦门大学化学生物学福建省重点实验室,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2011年第5期942-946,共5页Journal of Xiamen University：Natural Science

基　　金：福建省发改委产业技术开发项目(闽发改投资[2010]983号);厦门市科技计划项目(3502Z20093006)

摘　　要：对真养产碱杆菌(Ralstonia eutropha)H16累积聚-β-羟基丁酸酯(PHB)的发酵培养基组成进行了优化.在单因素实验的基础上,采用Plackett-Burman设计确定了碳源浓度和碳氮质量比两个显著影响真养产碱杆菌生长和累积PHB的因素.通过响应面设计法获得最优发酵培养基组成为:葡萄糖28.8 g/L,酵母粉0.2 g/L,碳氮质量比5,KH2PO4 1.0 g/L,Na2HPO4 0.3 g/L,MgSO4 1.0 g/L,CaCl2 0.1 g/L,ZnSO4 0.65 mg/L,FeSO4 0.1 mg/L,(NH4)6Mo7O240.3 mg/L,H3BO30.3 mg/L,pH 7.0.在此最优发酵条件下,细胞干质量浓度达到16.1 g/L,PHB的质量浓度达到7.9 g/L,糖对细胞的转化率为64%,对PHB的转化率为32%.The medium composition for poly-β-hydroxybutyrate（PHB） production by Ralstonia eutropha H16 was optimized inthis paper. Based on the single factor experiments, two most significant factors （initial carbon source concentration and initial C/N ratio）on Ralstonia eutropha H16 growth and PHB accumulation were confirmed by the Plackett-Burman design. According to response surface analysis,the optimal fermentation medium was composed as following：glucose 28.8 g/L, yeast extract O. 2 g/L,C/N ratio 5,KH2PO4 1.0 g/L,Na2 HPO4 0.3 g/L,MgSO4 1.0 g/L,CaCl2 0.1 g/L,ZnSO4 0.65 mg/L,FeSO4 0.1 mg/L,（NH4）6Mo7O24, 0.3 mg/L, H3 BO3 0, 3 mg/L, and pH 7.0. Under this optimal conditions, the biomass and the PHB concentration reach 16.1 g/L and 7.9 g/L,respectively. Conversion of glucose is 64% and 32% based on cells and PHB,respectively.

关 键 词：真养产碱杆菌 聚-β-羟基丁酸脂 可生物降解塑料 响应面 培养基 

分 类 号：Q815[生物学—生物工程]