四氯化碳诱导大鼠肝纤维化模型改良的研究  

Improved Rat Model of Liver Fibrosis induced by CCl_4

作　　者：邵佳亮[1] 万赞燕[1] 胡国信[1] 

机构地区：[1]南昌大学第一附属医院感染科,南昌330006

出　　处：《南昌大学学报（医学版）》2011年第7期40-42,51,F0003,共5页Journal of Nanchang University：Medical Sciences

基　　金：国家自然科学基金(30760230)

摘　　要：目的构建肝纤维化大鼠模型,并对经典四氯化碳(CCl4)复制模型方法进行适当改进。方法取Sprague-Dawley(SD)大鼠40只,雌雄各半,按随机数字表法分为A组10只、B组14只和C组16只。B组给予大鼠腹腔注射40%CCl4花生油溶液2 mL.kg-1,2次.周-1,同时给予普通全价饲料喂养(改良方法);A组为正常对照组,给予等量花生油腹腔注射,2次.周-1,同时给予普通全价饲料喂养;C组给予大鼠腹腔注射40%CCl4花生油溶液5 mL.kg-1,2次.周-1,同时给予添加10%猪油及2%胆固醇的饲料喂养(经典方法)。实验第8周末处死各组剩余大鼠:采用酶联免疫吸附法检测血清肝功能(ALT、AST、ALB、TP、A/G)水平;HE及Masson染色观察大鼠肝组织病理学变化。结果实验第8周B、C组均可见明显肝功能损害表现。B组可见部分标本肝脏假小叶形成,达到早期肝硬化,肝细胞少量脂肪变性,死亡率28.6%。C组均已形成明显肝硬化,弥漫性肝细胞脂肪变性,死亡率43.7%。结论低剂量CCl4诱导并给予普通饲料喂养可成功建立大鼠肝纤维化模型,并明显降低大鼠死亡率,提高了模型质量及实验效率。Objective To establish a rat model of liver fibrosis,and to improve the classical CCl4 induction method.Methods Forty SD rats（20 female and 20 male） were randomly divided into three groups.Group B（n=14） was given intraperitoneal injection of 2 mL·kg-1of 40% CCl4 solution（a mixture of CCl4 and peanut oil） twice a week and the feeding of normal complete diet.Group A（n=10） was given intraperitoneal injection of 2 mL·kg-1of peanut oil twice a week and the feeding of normal complete diet.Group C（n=16） was given intraperitoneal injection of 5 mL·kg-1of 40% CCl4 solution twice a week and the feeding of diet containing 10% lard and 2% cholesterol.Rats were killed at the end of 8 weeks,and the levels of serum ALT,AST,Alb,TP and A/G were determined by ELISA.The pathological changes in liver tissue were observed by HE and Masson staining.Results Liver function was damaged apparently by CCl4 injection.Some specimens in group B produced false flocculus and achieved the early stage of liver fibrosis,with fatty degeneration in a few liver cells.All specimens in group C had cirrhosis with diffuse hepatic steatosis.The mortality was 28.6% and 43.7% in group B and group C,respectively.Conclusion Liver fibrosis can be induced by injection of low doses of CCl4 and feeding of normal complete diet in rats.In addition,the improved method can reduce the mortality and improve the quality and efficiency of model experiment.

关 键 词：四氯化碳 肝纤维化模型 改良 动物 实验 大鼠 

分 类 号：R-332[医药卫生]