E-cadherin＼FAK、nm23-H1蛋白表达与声门上型喉癌颈淋巴结转移的相关因素研究  

Expression of E-cadherin, FAK and nm23-H1 proteins in carcinoma of supraglottic larynx with lymph node metastasis

作　　者：张靖华[1] 陈英武[1] 平金良 徐炜 顾栋烨 

机构地区：[1]湖州市中心医院耳鼻咽喉科,313000 [2]病理科

出　　处：《浙江医学》2011年第8期1130-1132,共3页Zhejiang Medical Journal

基　　金：湖州市自然科学基金项目（2008YZl5）

摘　　要：目的探讨E—cadherin、FAK、nm23-H1蛋白表达与声门上型喉癌颈淋巴结转移的关系，以利于临床上预估颈淋巴结转移情况。方法采用免疫组织化学Envision两步法检测70例声门上型喉癌中E—cadherin、FAK、nm23-H1的表达情况，结合患者临床资料分析3种免疫标志物（E—cadherin、FAK、nm23-H1蛋白）与颈淋巴结转移的相关性。结果E—cadherin及FAK蛋白表达与颈淋巴结转移均明显相关（P〈0．05）。nm23-H1蛋白表达与颈淋巴结转移无明显相关（P〉0．05）。E—cadherin及FAK蛋白均阳性表达与颈淋巴结转移显著相关（P〈0．01）。肿瘤分期、远处转移、病理分化程度均与颈淋巴结转移相关（均P〈0．01）。原发肿瘤分期T、组织分化G、FAK被纳入Logistic回归方程，利用该方程可得颈淋巴结无转移的准确率为75．6％．颈淋巴结有转移的准确率为82-7％，总体准确率为79．4％。结论声门上型喉癌转移与否与E—cadherin、FAK表达相关。与nm23-H1表达无明显相关，利用组织分化程度G、原发肿瘤分期T及FAK可以获得较高颈淋巴结转移的预计概率。Objective To investigate the expression of E-cadherin, FAK （Focal Adhesion Kinase） and nm23-H1 proteins in carcinoma of supraglottic larynx with cervical lymph node metastasis. Methods Expression of E-cadherin, FAK and nm23-H1 proteins were detected by Envision two-step immunohistochemical method in 70 cases of supraglottic laryngeal carcinoma. The relationship of E-cadherin, FAK and nm23-H1 expression with cervical lymph node metastasis was analyzed. Results E-cadherin and FAK were significantly correlated with cervical lymph node metastasis in supraglottic laryngeal cancer, but Nm23-H1 was not. Logistic regression showed that the diagnostic accuracy of combination of FAK expression, histological grade and T classification for cervical lymph node metastasis was 79.4%. Conclusions E-cadherin, FAK expression is significantly correlated with cervical node metastasis of supraglottic carcinoma. Combination with tissue differentiation G, primary tumor staging T and FAK expression can predict cervical lymph node metastasis with high accuracy.

关 键 词：E-CADHERIN蛋白 FAK蛋白 NM23-H1蛋白 声门上型喉癌颈淋巴结转移 

分 类 号：R734.2[医药卫生—肿瘤]