气相色谱-质谱法测定烟叶中5种致香成分  

GC-MS Determination of 5 Aromatic Components in Tobacco

作　　者：刘巍[1] 崔柱文 孔维松[1] 金永灿[1] 王岚[1] 陈永宽[1] 

机构地区：[1]云南烟草科学研究院云南省烟草化学重点实验室,昆明650106

出　　处：《理化检验（化学分册）》2011年第9期1095-1097,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：中国烟草总公司科技重大专项(110200902008);云南中烟工业公司项目(2009JC03)资助

摘　　要：用气相色谱-质谱法测定了烟叶中2-甲基四氢呋喃-3-酮（面包酮）、β-二氢大马酮、橙花基丙酮、β-紫罗兰酮和β-芳樟醇等5种致香成分的含量，以萘为内标物，采用内标校正曲线法进行分析。用DB-5MS石英毛细管柱、流量为lmL·min-1、程序升温（50℃～250℃）进行分析。采用电子轰击电离源（180℃，70eV）和选择离子监测，5种致香成分及内标的选择离子（m／z）为43，177，43，177，128和71。上述致香成分的线性范围分别为0．15～50，1．2～55，1．8～75，0．8～60，0．8～60mg·L-1，回收率为95．3％～106．6％。Contents of 5 aromatic components, i. e., 2-methyl-tetrahydrofuran-3-one, β-damascone, nerylacetone, β-ionone and β-1inalool, in tobacco were determined by GC-MS. Naphthalene was used as internal standard and the internal standard calibration curve was used in the determination. Quartz capillary chromatographic column DB-SMS was used for separation of the analytes with programmed temperature elevation from 50 ℃ to 250 ℃, with flow-rate of 1 mL · min- 1. Electron bombardment ionization （180℃, 70 eV） and SIM mode were adopted in MS, with the selected ions for the 5 components of （m/z） 43, 177, 43, 177, 128, 71. Ranges of linearity of the 5 components were found between 0. 15--50, 1. 2--55, 1. 8--75, 0. 8--60, 0. 8--60 mg ·L-1 respectively. Tests for recovery were made by addition of standard solutions of the 5 components to a tobacco sample, values of recovery found were in the range of 95.3%-106.6%.

关 键 词：烟叶 致香成分 气相色谱-质谱法 

分 类 号：O652.6[理学—分析化学]