缺氧诱导因子-1α在视网膜母细胞瘤中表达的区域性分布及其与血管内皮生长因子、Bax、Ki-67的关系  

The regional expression of hypoxia inducible factor-1α in retinoblastoma and its relationships with vascular endothelial growth factor, Bax and Ki-67

作　　者：岳晗[1] 钱江[1] 吴继红[2] 李纲[2] 毕颖文[3] 李霞萍[3] 

机构地区：[1]复旦大学附属眼耳鼻喉科医院眼科,上海200031 [2]复旦大学附属眼耳鼻喉科医院中心实验室,上海200031 [3]复旦大学附属眼耳鼻喉科医院病理科,上海200031

出　　处：《中华眼底病杂志》2011年第5期440-445,共6页Chinese Journal of Ocular Fundus Diseases

基　　金：上海市科委项目基金（074119636）

摘　　要：目的观察缺氧诱导因子-1α（HIF-1α）在视网膜母细胞瘤（RB）中表达分布的区域性及其与血管内皮生长因子（VEGF）、Ki67、Bax的关系。方法病理检查确诊的39例RB患儿的石蜡标本纳人研究。采用免疫组织化学EnVision法检测HIF-1α、VEGF、Bax和Ki-67在RB中的表达。按照肿瘤区域分为表面区、中央区、基底部、脉络膜区及子瘤5个区域，分析上述检测指标在肿瘤的表达、分布差异及相关性。结果39例RB标本中，HIF-1α表达阴性者10例，占25．6％；阳性者29例，占74．4％。其中，1＋者17例，占43．6％；2＋者12例，占30．8％。表面区、中央区、基底部、脉络膜区及子瘤区染色阳性者分别占71．1％、36．8％、84．2％、54．5％、82．1％。不同区域间阳性表达率分布比较，差异有统计学意义（x2=24．55，P〈O．001）。VEGF、Bax和Ki67表达阳性率分别为53．8％、66．7％、59．0％。其中，VEGF和Bax在不同区域的阳性表达率差异均有统计学意义（x2=26．77，22．79；P％0．001）；Ki-67在不同区域阳性表达率差异无统计学意义（x2=0．47，P=0．976）。HIF-1α与VEGF和Bax的表达呈正相关（rs=0．48，0．39；P=0．002，0．021），而与Ki-67的表达无明显相关（rs=0．09，P=0．606）。在不同区域，VEGF和Bax的表达均与HIF-1α有较好的一致性，即在肿瘤的表面区、基底部及子瘤区阳性率较高，在肿瘤的中央区和脉络膜区阳性率较低，而Ki-67与HIF-1α表达阳性率在不同区域无明显一致性。结论缺氧现象多分布在RB瘤体的边缘地带，且HIF-1α的表达在区域上与VEGF和Bax的表达成正相关关系。Objective To observe the regional expression of hypoxia inducible factor-1α （HIF-1α） in retinoblastoma and its relationships with vascular endothelial growth factor （VEGF）, Bax and Ki-67. Methods Immunohistoehemical study for HIF-1α, VEGF, Bax and Ki-67 was performed in 39 paraffinaeeous examples of retinoblastoma. Each pathological section was divided into five regions： the surface region, the central part, the bottom part, the choroidal region and seeding tumors. The expressions, correlations and distributional differences of these factors were all invested both integrally and regionally. Results In the 39 cases of retinoblastoma, 10 cases （25. 6%） were negative for HIF-1α; 29 cases （74.4%） were positive for HIF-1α, including 17 cases （43.6%） （＋）, 12 cases （30.8%） （＋＋）. Regionally, HIF-1α was positive in 71.1%, 36.8%, 84.2%, 54.5% and 82.1% of the cases in the surface region, the central part, the bottom part, the choroidal region and seeding tumors, respectively, which was statistically reliable （x2=24.55,P〈0. 001）. The positive rate of VEGF, Bax and Ki 67 was 53. 8%, 66.7% and 59.0%, respectively. In different regions, the positive rates of VEGF and Bax were different （Z2 =26.77, 22.79; P〈0. 001）, but there was no regional distinctions in the expression of Ki 67 （ 2 = 0. 47, P=0. 976）. Both the expression of VEGF and Bax had a positive correlation with that of HIF-1α （r,=0.48, 0.39; P=0.002, 0.021）, but there was no relationship between the expressions of Ki 67 and that of HIFla （rs=0.09, P=0.606）. Regionally, the expressions of VEGF, Baxand HIF-1α shared similar distributional features： positive rates were higher in the surface region, bottom part and seeding tumors, and were lower in the central part and choroidal region, which was different from the expression of Ki-67. Conclusion The anoxic zones are more likely to be located in the marginal parts in retinoblastoma, and the expressions of VEGF and Bax had a positive correlation with

关 键 词：视网膜母细胞瘤/治疗 缺氧诱导因子1 Α亚基 血管内皮生长因子类 抗药性 肿瘤 

分 类 号：R739.7[医药卫生—肿瘤]