葡萄糖转运蛋白1、缺氧诱导因子-1α和Ki-67在肝细胞癌中的表达及其相关性研究  

Study on the expression of Glutl, HIF-1α and Ki-67 in hepatoceHular carcinoma and their relationship

作　　者：周新木[1] 朱忆凌[1] 徐少杰[1] 孙洪鸣[1] 涂朝勇[2] 黎庆荣[1] 

机构地区：[1]温州医学院附属第五医院暨浙江省丽水市中心医院病理科,浙江省丽水323000 [2]温州医学院附属第五医院暨浙江省丽水市中心医院肝胆外科,浙江省丽水323000

出　　处：《中国医师杂志》2011年第8期1022-1025,共4页Journal of Chinese Physician

基　　金：浙江省丽水市科技局项目（20095915）

摘　　要：目的探讨葡萄糖转运蛋白1（Glutl）、缺氧诱导因子-1α（HIF-1α）和Ki-67在肝细胞癌中的表达及其相关性。方法运用组织芯片技术，通过免疫组织化学Envision法检测171例肝细胞癌组织、55例癌旁组织、22例正常肝组织中Glutl、HIF-1α和Ki-67的表达情况，并结合临床病理因素进行分析。结果Glutl、HIF-1α和Ki-67在171例肝细胞癌组织中的阳性表达率分别为15．2％、19．9％和66．1％，明显高于其在癌旁肝组织（1．8％、1．8％和5．5％）和正常肝组织（均阴性）中的表达率（P〈0．05）。Glutl和HIF-1α表达与肝细胞癌分化程度和TNM分期有关（P〈0．01，P〈0．05）；Ki-67表达与肝细胞癌分化程度有关（P〈0．01）。肝细胞癌组织中Glutl与HIF-1α的表达呈正相关（r1=0．553，P〈0．05）；Glutl、HIF-1α与Ki-67均呈正相关（r2=0．560，r3=0．613，P〈0．05）。结论Glutl、HIF-1α和Ki-67在肝细胞癌的发生发展中起着不同程度的作用，联合检测Glutl、HIF-1α和Ki-67可能有助于判断肝细胞癌的恶性程度、转移潜能及预后分析。Objective To explore the expression of Glutl, HIF-1α and Ki-67 in hepatocellular carcinoma （HCC） and their relationship between their expression and clinicopathological features. Methods Immunohistochemical study （EnVision method） for Glutl, HIF-1α and Ki-67 were performed on tissue microarray which consisted of 171 cases of HCC, 55 cases of adjacent non-neoplastic liver tissues, and 22 cases of normal liver tissues. Results The expression rate of Glutl ,HIF-1α and ki-67 in 171 cases of HCC was 15.2% , 19. 9% and 66. 1% , respectively, which was much higher than that in the adjacent non-neo- plastic liver tissues （ 1.8% ,1.8% and 5.5% ） and normal liver tissues（ all negative）. The expressions of Glutl and HIF-1αwere positively correlated with the differentiation degree of HCC and TNM stage （ P 〈 O. 01, P 〈 0. 05 ）. The expression of ki-67 was positively correlated with the differentiation degree of HCC. There was a significant positive correlation between the expressions of Glutl and HIF-let in HCC （ r1 =0. 553, P 〈0. 05）, the expressions of Glutl and HIF-1α were positively correlated with ki-67（ r2 =0. 560, r3 =0. 613, P 〈0. 05 ）. Conclusions Glutl, HIF-1α and ki-67 may play a role in the tumorigenesis and progression of HCC in some degree. Combined detection of Glutl, HIF-1α and Ki-67 may be helpful to judge the degree of malignancy and potential metastasis and evaluate the prognosis.

关 键 词：葡萄糖转运蛋白质类 易化性/代谢 缺氧诱导因子1/代谢 Ki-67抗原代谢 癌 肝细胞/代谢 

分 类 号：R735.7[医药卫生—肿瘤]