Caveolin-1对乳腺癌细胞系MCF-7增殖和存活的影响  

Effect of Caveolin-1 on Proliferation and Survival of MCF-7 Breast Cancer Cells

作　　者：陆瑞盛[1] 马骥[1] 张燕[1,2] 于玲莉[1,2] Ambre Spencer David Cluet Brian B.Rudkin 袁崇刚[1] 

机构地区：[1]华东师范大学生命科学学院 [2]Differentiation & Cell Cycle Group,Cellular Molecular Biology Laboratory,UMR 5239 CNRS/ENS Lyon/University of Lyon 1,IFR 128,Bioscience Lyon-Gerland.46,Allee d'Italie 69364 Lyon Cedex 07 [3]Differentiation & Cell Cycle Group Cellular Molecular Biology Laboratory,UMR 5239 CNRS/ENS Lyon/University of Lyon 1,IFR 128,Bioscience Lyon-Gerland.46,Allee d'Italie 69364 Lyon Cedex 07 [4]Differentiation & Cell Cycle Group

出　　处：《中国细胞生物学学报》2011年第9期983-987,共5页Chinese Journal of Cell Biology

基　　金：上海市国际合作(No.075407059)资助项目~~

摘　　要：该文研究窖蛋白(Caveolin-1)对乳腺癌细胞系MCF-7细胞增殖与存活的影响。运用蛋白质印迹方法(Western blot)检测发现,caveolin-1在5株不同细胞系均只有低表达。运用电穿孔转染方法在乳腺癌细胞系中高表达Caveolin-1,运用Western blot检测转染后Caveolin-1表达情况发现,转染后细胞内Caveolin-1表达上升,并具有生物活性。运用单核细胞直接细胞毒性测定法(MTT)检测发现,转染后乳腺癌细胞系MCF-7增殖速度降低。运用Western blot方法和免疫荧光(immunofluorescence)方法检测转染后细胞凋亡途径的变化,磷酸化的P38蛋白含量上升,Bax表达量明显上升。据此推测Caveolin-1抑制MCF-7细胞的增殖和存活,并诱导基于Bax途径的细胞凋亡。We Study the effect of Caveolin-1 on the proliferation and survival in MCF-7 breast cancer cells. A very low expression level was detected by Western blot in 6 breast cancer cell lines. Caveolin-1 expression increased dramatically after transient transfection and has its biological activity. The MTT assay showed that the proliferation rate of MCF-7 cells was decreased after the transfection of Caveolin-1. The cell apoptosis relative pro- tein was observed by Western blot and immunofluorescence. The quantity of phosphorylated P38 and the expression of Bax increased. We concluded that Caveolin-1 expression inhibited cells proliferation and survival and induces the Bax mediated cell apoptosis in MCF-7 breast cancer cells.

关 键 词：CAVEOLIN-1 乳腺癌 增殖和存活 

分 类 号：R737.9[医药卫生—肿瘤]