缺氧及沉默缺氧诱导因子-1α对BxPC-3细胞中RUNX3基因表达的影响  

Effect of hypoixa inducible factor-1α on RUNX3 gene expression of BxPC-3 cells under hypoxia

作　　者：潘定宇[1] 刘志苏[1] 刘立[1] 李震[1] 孔雕雕[1] 郭德良[1] 

机构地区：[1]武汉大学中南医院普通外科,430071

出　　处：《中华实验外科杂志》2011年第10期1723-1725,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察缺氧及应用小干扰RNA（siRNA）沉默缺氧诱导因子-1α（HIF-1α）基因对胰腺癌细胞株BxPC-3中RUNX3基因表达的影响。方法构建人RUNX3基因真核表达载体质粒pEGFP—RUNX3，CoCl2化学模拟肿瘤缺氧环境，检测缺氧以及siRNA沉默HIF-1α对BxPC-3细胞中RUNX3基因在mRNA和蛋白水平的影响。结果缺氧能使BxPC-3细胞中RUNX3基因的表达较常氧时显著升高[表达为（9．13±1．55），P〈0．05]，siRNA转染BxPC-3细胞后能显著下调HIF-1α蛋白表达（抑制率90％），并导致RUNX3基因的表达也受到明显抑制。结论缺氧促使BxPC-3细胞中HIF-1α在蛋白水平表达升高，缺氧时HIF-1α能上调RUNX3基因的表达水平。Objective To observe the expression of hypoixa inducible factor-1α （HIF-1α） and RUNX3 gene in BxPC-3 cells under hypoxia in vitro, and explore the regulation of RUNX3 gene expression by HIF-1α. Methods CoCl2 was used as a chemical hypoxia-inducible reagent to mimic tumor hypoxic mi- croenvironment. RUNX3 mRNA and protein expression was detected. The BxPC-3 cells were transfected with RNA interference （RNAi） originated by small interference RNA （siRNA）. The change of RUNX3 gene expression was observed after HIF-1α gene silencing. Results Under hypoxia for 24 h, RUNX3 in BxPC-3 cells was increased in mRNA and protein levels （P 〈 0. 01 ）. After siRNA transfection, the HIF-1α was down-regulated efficiently in BxPC-3 ceils （ inhibition ratio ： 90% ） , and RUNX3 gene was down-regu- lated as well. Conclusion Hypoxia can increase protein level of HIF-1α in BxPC-3 cells. HIF-1α up-reg- ulates the gene expression of RUNX3 under hypoxia.

关 键 词：RUNX3 缺氧诱导因子-1Α SIRNA 胰腺癌 缺氧 

分 类 号：R735.9[医药卫生—肿瘤]