Galectin-3在CD133＋肺腺癌细胞中表达增高诱导CD8＋T细胞凋亡  

Expression of Galectin-3 in CD133＋ pulmonary adenocarcinoma cells induced CD8＋ T cell apoptosis

作　　者：万黎[1] 王建军[1] 周雪峰[1] 赵峰[1] 范凯[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胸外科,武汉430022

出　　处：《中华实验外科杂志》2011年第10期1746-1748,共3页Chinese Journal of Experimental Surgery

基　　金：教育部博士点基金资助项目（20060487046）

摘　　要：目的检测肺腺癌CD133＋细胞中Galectin-3的表达。方法磁珠分选出10例患者肺腺癌中CD133＋细胞。流式细胞术检测分选效率，荧光实时定量聚合酶链反应（fqRT—PCR）和Westernblot检测CD133＋细胞中Galectin一3的表达，并在体外检测了CD133＋细胞上清诱导CD8＋T细胞凋亡的能力。结果流式细胞术结果表明磁珠分选出的细胞中CD133＋细胞数为90％。fqRT．PCR和Westernblot结果发现Galectin-3在CD133＋细胞中的表达量分别为CD133＋细胞中的1．24倍和1．5倍，差异有统计学意义（P〈0．05）。凋亡检测结果显示CD133＋细胞的上清诱导CD8＋T细胞凋亡率为（27．1±2．6）％，并且这种诱导凋亡的能力可被乳糖以及抗Galectin-3的抗体中和。结论Galectin-3在肺腺癌CD133＋细胞中高表达，并具有很强的生物学活性，在体外具有明显的诱导CD8＋T细胞凋亡的功能。Objective To investigate the expression and function of Galectin-3 in CD133＋ pulmonary adenocareinoma cells. Methods CD133 ＋ cells were separated by magnetic activated cell sorting （MACS） from reseeted pulmonary adenoearcinoma specimens of 10 patiens. The proportion of CD133 ＋ cells was measured by flow cytometry （FCM）. The expression of Galeetin-3 in CD133＋ or CD133- cells was quantitated by fluorescent quantitation real time-polymerase chain reaction （fqRT-PCR） and Western blotting. We also investigated whether the supernants of CD133＋ cells could mediate apoptosis of CD8 ＋ T cells in vitro. Results 90% cells separated by MACS were positive for CD133, which was proved by FCM. The expression of Gafectin-3 was 1.24 folds and 1.5 folds higher in CD133 ＋ cells than that in CD133 cells detected by fqRT-PCR and Western blotting. The supernants of CD133＋ cells more effectively induced apoptosis of CD8＋ T cells with apoptosis rate being （27. 1 ± 2. 6） % than those of CD133 cells with apoptosis rate being （ 10. 1 ±2. 2）% , which could be down-regulated by lactose and anti-CD133 monoclonal antibody. Conclusion Galectin-3 was highly expressed in CD133＋ pulmonary adenocareinoma cells, exerted strong biological activity, and could obviously mediate CD8 ＋ T cell apoptosis in vitro.

关 键 词：半乳凝集素3 CD133 肺腺癌 脱噬作用 

分 类 号：R734.2[医药卫生—肿瘤]