Survivin对PC-3细胞微小RNA表达的影响  

Influence of Survivin on microRNAs expression in prostate cancer PC-3 cells

作　　者：徐明[1] 单玉喜[1] 阳东荣[1] 薛波新[1] 崔勇[1] 

机构地区：[1]苏州大学附属第二医院泌尿外科,215004

出　　处：《中华实验外科杂志》2011年第10期1774-1776,共3页Chinese Journal of Experimental Surgery

基　　金：江苏省自然科学基金资助项目（BK2008167）;2008苏州市社会发展及医药项目

摘　　要：目的检测Survivin抑制后PC-3细胞微小RNA（miRNAs）表达谱的变化，探讨Survivin与相关miRNAs之间的关系。方法利用负载Survivin短发夹RNA（shRNA）重组腺病毒（pGSadeno．stirshRNA），体外转染PC-3细胞，96h后，收集细胞提取RNA，检测miRNA表达谱的变化。结果共有650个miRNAs表达信号在雄激素非依赖前列腺癌PC-3细胞中可以被检测到，有9个miRNAs的表达信号水平高于1000，其中的6个miRNAs表达谱同实，呤组比较差异有统计学意义（P〈0．01）。实验组与空白对照组比较有75个miRNAs表达差异有统计学意义（P〈0．01），其中42个表达上调、33个表达下调；阴性对照组与实验组比较有27个miRNAs表达差异有统计学意义（P〈0．01），其中22个表达上调、5个表达下调；实验组与空白对照组及阴性对照组比较均表达上调的miRNAs有15个，表达下调的miRNA有1个。结论抑制PC-3细胞中Survivin的表达对miRNAs表达谱有明显影响，为明确相关miRNAs在PC-3细胞中的作用提供新线索。Objective The microRNA（miRNA） ;xp;;ssi;n ;rofile was detected after Survivin mRNA was inhibited in PC-3 ceils and the potential relationship of Survivin and related miRNA was predic- ted. Methods The prostate cancer PC-3 cells were transfected in vitro by recombinant adenovirus, pGSad- eno-sur short hairpin RNA （shRNA） , which loading Survivin shRNA. Ninety-six h after transfection, the cells were collected, small RNAs were isolated and the expression profiles were analyzed by microarray technique. Results In total, the signals of 650 miRNAs were detected in PC-3 cells. Of 9 miRNAs with signal levels beyond 1000, the expression profiles of 6 miRNAs had significant difference between control group and experimental group （P 〈 0. 01 ）. A total of 75 miRNAs showed significant difference in the expression patterns between experimental group and blank group （ P 〈 0. 01 ） , including 42 significantly upregulated, and 33 significantly down-regulated （P 〈 0.01 ）. But between experimental group and negative control group, there was significant difference in the expression of 27 miRNAs （P 〈 0. 01 ）, including 22 up-regulated and 5 down-regulated. Sixteen miRNAs had significant difference in the expression patterns between experimental group and blank group or negative control group, including 15 up-regulated and 1 down-regulated （ P 〈 0. 01 ）. Conclusion The miRNAs expression profiles were influenced markedly when Survivin mRNA was inhibited in PC-3 cells, which can support a novel clue for uncovering related miRNAs＇ mechanism in PC-3 cells.

关 键 词：MIRNA 前列腺癌 SURVIVIN 癌基因 

分 类 号：R737.25[医药卫生—肿瘤]