HPV16整合状态与宫颈癌发生的相关性研究  

Study on the correlation between integrated state of HPV-16 and the occurrence of cervical cancer

作　　者：李科珍[1] 金鑫[1] 方勇[1] 艾继辉[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,湖北武汉430030

出　　处：《中国妇幼保健》2011年第27期4241-4244,共4页Maternal and Child Health Care of China

基　　金：国家自然科学基金资助项目〔81001152〕;湖北省自然科学基金资助项目〔2010CDB09403〕

摘　　要：目的:通过检测宫颈癌患者中HPV-16的患病率和不同级别宫颈病变中HPV存在状态的研究探讨HPV-16整合在宫颈癌发生发展中的作用。方法:运用HPV通用型引物及HPV-16型特异型引物相继检测宫颈癌标本中HPV-16感染的患病率,采用定量PCR的方法检测HPV-16感染的112例宫颈癌标本、151例CINI标本、246例CINⅡ标本和120例CINIII标本中HPV的存在状态,分析HPV-16整合状态与宫颈病变严重程度的相关性。结果:在宫颈癌患者中HPV-16感染患者有112例,约占宫颈癌总患者数的56.57%;在112例感染HPV-16的宫颈癌标本中,HPV-16单纯整合感染的病例超过半数共有73例,阳性率达到65.18%(73/112);在CINI、CINⅡ、CIN III患者中,HPV-16单纯整合型感染率分别为7.95%、23.58%和39.17%,宫颈癌患者的HPV-16整合率与CINI、CINⅡ、CINIII患者相比差异有统计学意义。结论:HPV-16是宫颈癌患者中最常见的病毒感染类型,在感染HPV-16的患者中HPV的整合状态与宫颈病变严重程度明显呈正相关。如能在HC-ⅡHPVDNA检测的基础上辅以HPV的分型检查及病毒整合状态的检测将更加有利于宫颈癌前病变患者的预后判断和增加宫颈癌筛查的准确性。Objective：To explore the role of HPV-16 integration in the occurrence and development of cervical cancer by measuring the prevalence of HPV-16 in patients with cervical cancer and the existential state of HPV in cervical lesions of different grades. Methods：A universal primer of HPV and a specific primer of HPV-16 were used to detect the infection rate of HPV-16 in cervical cancer samples,quantitative PCR was adopted to detect the existential state of HPV in HPV-16 infected samples（including 112 samples of cervical cancer,151 samples of CIN Ⅰ,246 samples of CIN Ⅱ and 120 samples of CIN Ⅲ）;the correlation between integrated state of HPV-16 and the severity of cervical lesions was analyzed. Results：Among the patients with cervical cancer,112 patients were infected by HPV-16,accounting for 56.57% of the total patients with cervical cancer;among 112 HPV-16 infected patients with cervical cancer,73 patients were found with simple integrated infection of HPV-16,the positive rate was 65.18%（73/112）;the infection rates of simple integrated HPV-16 in the patients with CIN Ⅰ,CIN Ⅱ and CIN Ⅲ were 7.95%,23.58% and 39.17%,respectively;there was significant difference in the infection rate of simple integrated HPV-16 between the patients with cervical cancer and the patients with CIN Ⅰ,CIN Ⅱ and CIN Ⅲ. Conclusion：HPV-16 is the most common type of viral infection in patients with cervical cancer,among the patients with HPV-16 infection,the integrated state of HPV is positively correlated with the severity of cervical lesions.Assisted HPV typing test and detection of viral integrated state will be more helpful to predict the prognosis of patients with precancerous lesions of uterine cervix and increase the accuracy of cervical cancer screening on the basis of HC-II HPV DNA detection.

关 键 词：人乳头状瘤病毒 宫颈癌 分型 存在状态 整合 

分 类 号：R737.33[医药卫生—肿瘤]