体外膜肺氧合对内毒素诱导大鼠急性肺损伤后肺气体交换的影响  

The effects of extracorporeal membrane oxygenation on gas exchanges of acute lung injury induced by endotoxin

作　　者：陈永梅[1] 龚兴瑞[1,2] 

机构地区：[1]湖北医药学院附属太和医院检验科,十堰442000 [2]湖北医药学院附属太和医院麻醉科

出　　处：《陕西医学杂志》2011年第9期1116-1118,共3页Shaanxi Medical Journal

摘　　要：目的:观察体外膜肺氧合(ECMO)对内毒素诱导大鼠急性肺损伤(ALI)后肺气体交换的影响。方法:选择健康成年SD大鼠40只,随机分为对照组、模型组(LPS组)、体外膜肺氧合组(ECMO组)。LPS组和ECMO组在麻醉后给予脂多糖(LPS)0.5mg/kg静脉注射;ECMO组在LPS注射1h后开始转流体外循环,转流时间为2h,对照组注射生理盐水。观察时间点为:T0:LPS注射前;T1:LPS注射后1h;T2:LPS注射后2h;T3:LPS注射后3h;T4:LPS注射后5h。分别在5个时间点观察3组大鼠的血压、心率、呼吸频率、动脉血氧分压(PO2)、动脉血CO2分压(PCO2),5h后处死大鼠测量肺组织湿(干)重量比值。结果:T2、T3、T4时刻对照组、ECMO组与模型组相比血压、心率较稳定,PO2较高,PCO2较低(P<0.05);大鼠处死后,对照组、ECMO组术后肺组织湿(干)比值较小(P<0.05)。结论:内毒素导致急性肺损伤时,通过ECMO治疗可减轻肺水肿和呼吸窘迫症状,改善机体缺氧和CO2蓄积。Objective：To observe the effects of extracorporeal membrane oxygenation（ECMO） on gas exchange of acute lung injury（ALI） induced by endotoxin.Methods： 40 healthy SD rats were randomly divided into three groups： control group,model group（LPS） and ECMO group.Both model group and ECMO group were received Lipopolysaccharide 0.5mg/kg intravenous injection after anesthesia induction for setting up acute lung injury models while the control group received saline.The observation time were T0： before Lipopolysaccharide injection;T1：1 hour after Lipopolysaccharide injection;T2：2 hours after Lipopolysaccharide injection;T3： 3 hours after Lipopolysaccharide injection;T4：5 hours after Lipopolysaccharide injection.The observation included blood pressure,respiratory rate,PO2,PCO2;The ratio of dry/wet of lung tissue was calculated when the rats were killed at T4.Results：the control group and ECMO group caused minor change of blood pressure,heart rate,respiratory rate,PO2 and PCO2during T2,T3,T4 when compared with model group,the difference was statistically significant（P〈0.05）;the control group and ECMO group had a lower ratio of wet/dry（P〈0.05）.Conclusion：ECMO can eliminate surplus CO2 accumulation,improve PO2, ameliorate lung edema and symptoms of respiratory distress of acute lung injury induced by Lipopolysaccharide.

关 键 词：呼吸窘迫综合征/化学诱导 呼吸窘迫综合征/病理生理学 体外膜氧合作用 模型 动物 大鼠 

分 类 号：R363.23[医药卫生—病理学]