金银花脂溶性成分的气相-质谱联用分析  

GC-MS analysis of liposoluble contents from the flowers of Flos Lonicerae japonicae

作　　者：王静[1] 李峰[1] 陈永新[1] 刘亚鲁[1] 

机构地区：[1]山东中医药大学药学院,山东济南250355

出　　处：《山东中医药大学学报》2011年第5期450-452,共3页Journal of Shandong University of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(编号:2007CB512601);山东省科技发展计划课题(编号:2006GG2202017;2006GG3202003)

摘　　要：目的:探讨金银花脂溶性成分的组成及其研究意义。方法:采用索氏提取法提取脂溶性成分,用气相毛细管柱进行分析,归一化法测定其相对含量,并用气相色谱-质谱联用(GC-MS)法鉴定其化学成分。结果:检出37个色谱峰,鉴定出25个化合物,占总组分的98.87%。脂溶性成分主要为脂肪酸和烃类,其中10种脂肪酸占总脂溶性成分的46.18%,9种烃类化合物占总脂溶性成分的33.88%;酯类成分和植物甾醇为次要成分,分别占总脂溶性成分的9.21%和8.39%。结论:金银花中的脂溶性成分种类丰富并具有一些药理活性,气相-质谱联用技术能够有效地分析金银花中脂溶性成分。Objective：Analyze the chemical constituents of the liposoluble contents from Flos Lonicerae japonicae and its significance.Methods：The liposoluble contents of Flos Lonicerae japonicae were extracted by Soxet extraction and separated with GC capillary column.The relative content of every compound was determined by normalization method and the chemical compositions were evaluated by GC-MS.Results：Total 37 kinds of chromatographic peaks were isolated and 25 compounds were identified,accounting for 98.87% of the total liposoluble contents in Flos Lonicerae japonicae.The essential components of Flos Lonicerae japonicae were fatty and acid hydrocarbon compounds,46.18% of the total liposoluble contents were 10 kinds of fatty acid,33.88% of the total liposoluble contents were 9 kinds of hydrocarbon compounds.Phytosterol and esters contents were the secondary composition,accounting for 9.21% and 8.39%.Conclusion：There are many kinds of liposoluble contents in Flos Lonicerae japonicae and they also have some certain pharmacological activity.GC-MS method can analyze the liposoluble contents of Flos Lonicerae japonicae effectively.

关 键 词：金银花 脂溶性成分 气相色谱-质谱 

分 类 号：R284.2[医药卫生—中药学]