鲁米诺-过氧化氢化学发光体系分析环吡酮胺及其制剂  

Analysis of ciclopirox olamine with luminol-H_2O_2 chemiluminescence system

作　　者：安静[1] 张福成[2] 倪美萍[1] 卢彦芳[1] 蒋晔[1] 

机构地区：[1]河北医科大学药学院,石家庄050017 [2]空军总医院药学部,北京100036

出　　处：《分析试验室》2011年第10期98-101,共4页Chinese Journal of Analysis Laboratory

摘　　要：在碱性介质中,环吡酮胺对H2O2氧化鲁米诺化学发光体系有较强的增敏作用,据此建立了流动注射-化学发光测定环吡酮胺的新方法,显著地提高了检测灵敏度。在优化的试验条件下,发光信号的增敏值(ΔI)与环吡酮胺溶液的浓度ρ在0.25～4.0μg/mL范围内线性关系良好,检出限为0.06μg/mL。对浓度为1μg/mL环吡酮胺进行11次平行测定,相对标准偏差(RSD)为1.9%。方法可直接进样分析,分析频率为100/h。该方法已用于环吡酮胺制剂溶出度的研究。A simple chemiluminescence method for the determination of ciclopirox olamine with flow injection technique was proposed,which was based on enhancing the chemiluminescence from H2O2 and luminol in alkaline medium.The linear range of the calibration curve was 0.25~4.0 μg/mL,and the detection limit was 0.06 μg/mL.The RSD of 1 μg/mL ciclopirox olamine was 1.9 %（n = 11）.The method was simple and fast with the sampling frequency of the system of 100/h.It has been applied to the dissolution testing of ciclopirox olamine in pharmaceutical samples.

关 键 词：化学发光 流动注射 鲁米诺 环吡酮胺 溶出度测定 

分 类 号：O657.3[理学—分析化学]