维生素K_3对松浦镜鲤体成分、肉质和抗氧化能力的影响  

Effects of Vitamin K_3 on Body Composition,Meat Quality and Antioxidant Capacity of Songpu Mirror Carp

作　　者：王洋[1,2] 徐奇友[2] 许红[2] 王常安[2] 石连玉[2] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]中国水产科学研究院黑龙江水产研究所,哈尔滨150070

出　　处：《动物营养学报》2011年第6期1058-1064,共7页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：现代农业产业技术体系建设专项资金(Nycytx-49-10);黑水研基本科研专项(2009HSYZX-YZ-01)

摘　　要：本试验旨在研究饲料中添加不同水平的维生素K3对松浦镜鲤体成分、肉质和抗氧化能力的影响。在以豆粕和鱼粉为蛋白质源的基础饲料（维生素K3含量为0．54mg／kg）中分别添加0、5、10、20、40、80和2000mg／kg的维生素K3（甲萘醌烟酰胺亚硫酸盐），配制成7种试验饲料。选取初始体重为（2．58±0．16）g的松浦镜鲤幼鱼525尾，随机分为7组，每组设3个重复，每重复放养25尾，在水温24℃、溶氧7．46～8．61mg／L条件下饲喂60d。结果表明，添加不同水平的维生素K。对松浦镜鲤的体成分（水分、粗蛋白质、粗脂肪、灰分含量）以及肝胰脏中丙二醛（MDA）含量均无显著影响（P〉0．05）；0mg／kg组的剪切力显著小于其他各组（P〈0．05）；10mg／kg组的pH24h显著小于其他各组（P〈0．05），滴水损失显著大于0和5mg／kg组（P〈0．05），肝胰脏超氧化物歧化酶（SOD）活性显著大于0和5mg／kg组（P〈0．05），肝胰脏还原型谷胱甘肽（GSH）含量显著大于2000mg／kg组（P〈0．05）；10和20mg／kg组的肝胰脏总抗氧化能力（T—AOC）显著大于0、80和2000mg／kg组（P〈0．05），但二者差异不显著（P〉0．05）。通过折线模型对饲料中维生素K3添加水平和肝胰脏中T—AOC进行一元回归分析，得出T—AOC最高时的维生素K3的添加量为16．89mg／kg。由此得出，饲料中添加维生素K。可提高松浦镜鲤肌肉的剪切力，降低肌肉的系水力；不添加或过量添加维生素K3均会降低松浦镜鲤的抗氧化能力，其适宜添加量为16．89mg／kg。This experiment was conducted to study the effects of different dietary vitamin K3 levels on body composition, meat quality and antioxidant capacity of Songpu mirror carp. A total of 525 juvenile Songpu mirror carp with average body weight of （2.58 ±0.16） g were randomly allocated into 7 groups with 3 replicates per group and 25 fish per replicate. The fish in 7 groups were fed the basal diet （ vitamin K3 content was 0.54 mg/kg） supplemented with 0, 5, 10, 20, 40, 80 and 2 000 mg/kg vitamin K3 （ menadione nicotinamide bisulfite, MNB ）, respectively. Under the conditions of water temperature 24 ℃ and dissolved oxygen 7.46 to 8.61 mg/L, the experiment lasted for 60 days. The results showed as follows ： different levels of vitamin Ka supplementation could not affect the body composition （moisture, crude protein, ether extract and ash contents） and the hepatopancreas malonyldiadehyde （MDA） content of Songpu mirror carp （ P 〉 0.05 ）. The shearing force of Songpu mirror carp in 0 mg/kg group was significantly lower than that in other groups （ P 〈 0.05 ）. In 10 mg/kg group, the pH24 h was significantly lower than that in other groups （ P 〈 0.05 ） , but the drip loss and superoxide dismutase （SOD） activity in hepatopancreas were significantly higher than those in 0 and 5 mg/kg groups （ P 〈 0.05 ） , and the glutathione （GSH） content in hepatopancreas was significantly higher than that in 2 000 mg/kg group （ P 〈 0.05 ） ; the hepatopancreas total antioxidant capacity （T-AOC） in 10 and 20 mg/kg groups were significantly higher than those in 0, 80 and 2000 mg/kg groups （P 〈 0.05 ）, but no significant difference was found between 10 and 20 mg/kg groups. The linear regression result showed that the requirement for vitamin K3 of Songpu mirror carp which had the highest T-AOC was 16.89 mg/kg diet. In conclusion, adding vitamin Ka in diet can improve the muscle shearing force and reduce the muscle ca- pacity of holding water of Songpu mirror carp. Whether no

关 键 词：松浦镜鲤 维生素K3 体成分 肉质 抗氧化能力 

分 类 号：S963[农业科学—水产养殖]