瘤胃灌注大豆小肽对肉牛瘤胃发酵的影响  

Infusion of Soybean Small Peptides into Rumen:Effects on Rumen Fermentation of Beef Cattle

作　　者：王文娟[1,2] 万发春[3,2] 杨维仁[1] 宋恩亮[3,2] 刘晓牧[3,2] 谭秀文[3,2] 刘桂芬[3,2] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]山东省畜禽疫病防治与繁育重点实验室,济南250100 [3]山东省农业科学院畜牧兽医研究所,济南250100

出　　处：《动物营养学报》2011年第8期1324-1331,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：现代农业(肉牛)产业体系专项经费(MATS-BeefCattleSystem);公益性行业农业科研专项经费课题(nyhyzx07-036-01;ny-hyzx07-036-05)

摘　　要：本试验旨在探讨瘤胃灌注不同水平大豆小肽对肉牛瘤胃发酵指标和瘤胃微生物的影响。选用4头安装永久性瘤胃瘘管的鲁西黄牛阉牛,采用4×4拉丁方试验设计,分别瘤胃灌注0、100、200和300 g/d的大豆小肽。采集瘤胃液,测定瘤胃发酵指标,实时定量PCR法测定瘤胃微生物相对含量。结果表明,灌注大豆小肽对瘤胃液pH平均值无显著影响(P>0.05),显著提高了瘤胃液氨态氮(NH3-N)与微生物蛋白(MCP)平均浓度(P<0.05);灌注大豆小肽显著提高了瘤胃液总挥发性脂肪酸(TVFA)的浓度(P<0.05),与0 g/d组相比,200 g/d组和300 g/d组丙酸百分含量显著增加(P<0.05),100 g/d组和200 g/d组乙酸百分含量显著降低(P<0.05),300 g/d组丁酸含量显著降低(P<0.05);灌注大豆小肽显著降低了乙酸/丙酸(P<0.05);灌注小肽显著提高了瘤胃液中溶纤维丁酸弧菌含量(P<0.05)。可见,瘤胃灌注大豆小肽可促进MCP的合成、提高溶纤维丁酸弧菌的含量、改善瘤胃代谢。The experiment was conducted to investigate the effects of infusion of different levels of soybean small peptides（SSP） on rumen fermentation and rumen microbes in Luxi cattle.Four heads of Luxi cattle fixed with permanent ruminal cannula were used in a 4×4 Latin square design.Cattle were infused SSP by 0,100,200 and 300 g/d,respectively.Ruminal fluid was collected for the analysis of rumen fermentation indices,as well as the relative content of rumen microbes by real-time PCR.The results showed as follows： the mean of ruminal pH was not significantly affected by SSP infusion（P0.05）,the means of NH3-N and MCP concentrations were significantly increased（P0.05）;the concentration of total volatile fatty acid（TVFA） was significantly increased by SSP infusion（P0.05）,compared with those in 0 g/d group,the proportions of propionate in 200 g/d group and 300 g/d group were increased significantly（P0.05）,while the proportions of acetate in 100 g/d group and 200 g/d group,as well as the proportions of butyrate in 300 g/d group were decreased significantly（P0.05）;the ratio of acetate to propionate was significantly decreased by SSP infusion（P0.05）;the relative content of Butyrivibrio fibrisolvens was significantly increased by SSP infusion（P0.05）.In conclusion,infusion of SSP can promote microbial protein synthesis,increase Butyrivibrio fibrisolvens content and improve rumen metabolism.

关 键 词：大豆小肽 肉牛 瘤胃发酵 瘤胃微生物 

分 类 号：S823[农业科学—畜牧学] S816.4[农业科学—畜牧兽医]