丙戊酸钠诱导人肝癌细胞株Bel-7402凋亡  

Sodium valproate induces apoptosis of human liver cancer Bel-7402 cell line

作　　者：赵慧慧[1] 

机构地区：[1]东南大学附属南京第二医院肿瘤科,南京市210003

出　　处：《江苏医药》2011年第17期2004-2006,共3页Jiangsu Medical Journal

摘　　要：目的探讨丙戊酸钠(VPA)对人肝癌Bel-7402细胞增殖抑制和诱导凋亡作用。方法采用四甲基偶氮唑蓝(MTT)比色法检测细胞生长的抑制作用,流式细胞术检测细胞凋亡率,免疫组化和Western blot印迹方法检测血管内皮生长因子(VEGF)蛋白的表达水平。结果 VPA对Bel-7402细胞的生长抑制作用呈现时间和剂量依赖性;经1、2和4 mmol/L VPA作用细胞72 h后,细胞的凋亡率由处理前的(2.78±0.32)%,上升为(8.79±0.53)%、(18.65±1.02)%和(36.41±1.93)%;VEGF蛋白的表达明显呈浓度依赖性降低(P<0.05)。结论 VPA对Bel-7402细胞有显著的增殖抑制及诱导凋亡作用,其作用机制可能与下调VEGF的表达有关。Objective To investigate the effects of sodium valproate in inhibiting cell proliferation and inducing apoptosis of human liver cancer Bel-7402 cell line.Methods The cell proliferation was determined by MTT assay,cell apoptosis was analyzed by flow cytometry,the expression of vascular endothelial growth factor（VEGF） protein was analyzed by immuno-cytochemistry and Western blot.Results Sodium valproate inhibited the proliferation of Bel-7402 cells in a does-and time-dependent manner.After treated with 1,2 and 4 mmol/L VPA for 72 hours,the percentage of apoptotic cells increased from（2.78±0.32）% to（8.79±0.53）%,（18.65±1.02）% and（36.41±1.93）%,respectively,and the expression of VEGF protein was down-regulated in a dose-dependent manner（P〈0.05）.Conclusion Sodium valproate not only inhibits cell proliferation but also induces apoptosis of Bel-7402 cells,possiblly through down-regulating the expression of VEGF.

关 键 词：丙戊酸钠 BEL-7402细胞 凋亡 血管内皮生长因子 

分 类 号：R735[医药卫生—肿瘤]