垂体肿瘤转化基因在大肠正常黏膜、腺瘤及大肠癌组织中的表达及意义  

Expression of PTTG in Normal Mucosa,Adenoma and Colorectal Carcinoma

作　　者：申兴斌[1] 段惠佳[2] 赵杨[1] 张古林 

机构地区：[1]承德医学院附属医院病理科,河北承德067000 [2]中国人民解放军281医院心内科 [3]承德市兴隆县医院病理科

出　　处：《肿瘤防治研究》2011年第9期1042-1045,共4页Cancer Research on Prevention and Treatment

基　　金：河北省科技厅资助课题(08276101D-10)

摘　　要：目的探讨垂体肿瘤转化基因(PTTG)的表达与大肠癌发生、发展的关系。方法采用反转录聚合酶链式反应(RT-PCR)技术及免疫组织化学法检测20例大肠正常黏膜、20例大肠腺瘤、80例大肠癌组织中PTTG mRNA及蛋白的表达水平。结果大肠癌组织中PTTG mRNA及蛋白的表达量明显高于大肠腺瘤和正常黏膜组织(P均<0.01),大肠腺瘤组织亦高于正常黏膜组织(P<0.01),且PTTGmRNA及蛋白的表达量与大肠癌浸润深度、淋巴结转移呈正相关。结论 PTTG的高表达在大肠癌的发生、发展过程中起到重要作用,可作为判断大肠癌发生发展的预测因素。Objective To explore the role of PTTG expression in the occurrence and development of colorectal carcinoma.Methods RT-PCR technique was used to examine mRNA expression of PTTG and immunohistochemical staining was used to examine protein expression of PTTG in 20 cases of normal mucosa,20 cases of adenoma and 80 cases of colorectal carcinoma tissues.Results Expression of PTTG at mRNA and protein appeared an upward tendency in the order from normal mucosa and adenoma to colorectal carcinoma and the differences was significant between any two groups（P0.01）.Expression of PTTG at mRNA and protein was also positively correlated with depth of invasion,lymph node metastasis（P0.05 and P0.01,respectively）.Conclusion The high expression of PTTG may play an important role in the occurrence and development of colorectal cancer.

关 键 词：大肠癌 垂体肿瘤转化基因 反转录聚合酶链式反应 免疫组织化学 

分 类 号：R735.34[医药卫生—肿瘤]